摘要
目的：观察异丙嗪（ｐｒｏｍｅｔｈａｚｉｎｅ，ＰＭＺ）对家兔ＥＧＴＡ性发热的影响并探讨其作用机制。方法：侧脑室和静脉给药。用Ｆｕｒａ－２荧光分光光度法测定细胞内游离钙浓度（［Ｃａ２＋］ｉ）。结果：（１）侧脑室灌注０６μｍｏｌＥＧＴＡ引起家兔明显的发热反应，侧脑室灌注０６μｍｏｌＥＧＴＡ２０ｍｉｎ后，静脉注射ＰＭＺ（５ｍｇ／ｋｇ）明显抑制ＥＧＴＡ引起的结肠温度上升，其３ｈ发热反应指数明显低于侧脑室灌注０６μｍｏｌＥＧＴＡ２０ｍｉｎ后静脉注射生理盐水（ＮＳ）组。而侧脑室灌注人工脑脊液（ＡＣＳＦ）２０ｍｉｎ后静脉注射ＰＭＺ（５ｍｇ／ｋｇ）组家兔结肠温度明显低于ＡＣＳＦ＋ＮＳ对照组。（２）体外实验发现，向下丘脑细胞悬液中加入终浓度为０７４ｍｍｏｌ／Ｌ的ＰＭＺ，下丘脑细胞［Ｃａ２＋］ｉ从（１５９２±１８８）ｎｍｏｌ／Ｌ升高到（５３３７±９０１）ｎｍｏｌ／Ｌ（Ｐ＜００５）。结论：ＰＭＺ诱导体温调节中枢神经细胞［Ｃａ２＋］ｉ升高可能是ＰＭＺ抑制家兔ＥＧＴＡ性发热的中枢机制之一。
AIM:To investigate the effect of promethazine(PMZ)on egtazic acid-induced fever in rabbits and its mechanism.METHODS:Egtazic acid was administrated intracerebroventricularly,PMZ was given intravenously.Free Ca 2+ intracellular concentrations([Ca 2+ ] i)was measured by Fura-2 fluorometry.RESULTS:(1)0 06 μmol egtazic acid intracerebroventricularly infused induced a rise in colonic temperature,when PMZ(5 mg/kg) was administrated intravenously 20 min after the infusion of egtazic acid,it reduced the febrile responses of rabbits to egtazic acid significantly (0.06 μmol) but without complete suppression.PMZ administrated alone caused a marked decrease in colonic temperature.(2)PMZ (0.74 mmol/L) increased [Ca 2+ ] i in hypothalamus cells from(159.2±18 8)nmol/L to(533 7±90 1)nmol/L ( P <0 05).CONCLUSION:PMZ possesses antipyretic effect on egtazic acid-induced fever in the rabbit and raises the possibility that the antipyretic actions of PMZ are mediated through the increase of [Ca 2+ ] i incuced by PMZ.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
1999年第3期222-224,共3页
Chinese Journal of Pathophysiology
