摘要
目的:观察中药蜈蚣败毒饮对小鼠尾部鳞片表皮颗粒层形成及血清IL-2水平的影响,初步探讨其治疗银屑病的作用机理。方法:取雌雄各半昆明种小鼠50只,随机分成5组。中药组分别给予低、中、高浓度蜈蚣败毒饮灌胃,阳性对照组给予复方青黛丸悬浊液灌胃,生理盐水组给予生理盐水灌胃。观察蜈蚣败毒饮对小鼠尾部鳞片表皮颗粒层形成的影响,并测定IL-2含量。结果:阳性对照组、中药各剂量组与生理盐水组比较颗粒层细胞均有所增多。蜈蚣败毒饮中、高剂量组颗粒层细胞增多更为明显,能够显著促进小鼠尾部鳞片中颗粒层形成,与阳性对照组比较有显著性差异(P<0.05或P<0.01)。蜈蚣败毒饮能降低小鼠血清IL-2的水平,与生理盐水组比较,有显著性差异(P<0.01);且蜈蚣败毒饮高剂量组的效果优于复方青黛丸及各剂量组,有显著性差异(P<0.05)。结论:蜈蚣败毒饮抗银屑病的作用可能与改善银屑病表皮细胞角化不全及降低血清IL-2水平有关。
Objective ： Observe the effect of Centipede Du Bai Yin on the Formation of Mouse - tail Epidermis Granular Layer and the Effect on the Level of IL -2 in Serum. Preliminary study on the treatment mechanism in curing Psoriasis. Methods ： Fifty Kun Ming Mouse, half male and half female, were divided into five groups randomly. The experiment group were administered respectively to low, medium and high groups by gavage. Positive control group was given liquid suspension of compound Qingdai pill by gavage. Physiological saline group was given physiological saline by gavage. Observe the effect of Centipede Du Bai Yin on the Formation of Mouse - tail Epidermis Granular Layer and determinate the content of IL - 2 according to the introduction of ELISA kit. Results ： The granular layer cell of positive control group, all dose of experimental groups were def- initely increased compared with physiological saline group. Centipede Du Bai Yin medium and high dose group increased more obviously, and can significantly promote the formation of mouse - tail epidermis granular layer, which has significant difference（P 〈 0. 05 or P 〈 0. 01 ）. Centipede Du Bai Yin can decrease the Level of IL -2 in mice Serum, which has significant difference compared with physiological saline group（ P 〈 0. 01 ）; And the effect of medium and high groups were better than compound qingdai pill as well as other dose groups, which has significant difference（ P 〈0. 05 ）. Conclusion： The effect of Centipede Du Bai Yin Anti -psoriasis may be related to improving Psoriasis epidermal cell parakeratosis and decreasing the level of IL - 2 in Serum.
出处
《中医药信息》
2010年第4期45-48,共4页
Information on Traditional Chinese Medicine
基金
黑龙江省中医管理局(HZ2005-30)
关键词
银屑病
白介素-2
颗粒层细胞
psoriasis
interleukin - 2
granular layer cell
