摘要
采用结扎大鼠双侧颈总动脉合并低血压后再恢复供血造成大鼠脑缺血再灌注模型，运用 斑点杂交技术，观察大鼠全脑缺血再灌注中海马ｃ－ｆｏｓ ｍＲＮＡ表达的动态变化，并观察小檗碱 （Ｂｅｒ）的作用。结果表明，大鼠全脑缺血３０ ｍｉｎ后再灌注１０～１８０ｍｉｎ，海马ｃ－ｆｏｓ ｍＲＮＡ出现一过 性高表达，１ｈ达高峰，为假手术组的５．１倍。Ｂｅｒ２０ｍｇ／（ｋｇ·ｄ）×５ｄｉｐ显著抑制脑缺血诱导的 ｃ－ｆｏｓ高表达，且明显升高ＳＯＤ及ＧＳＨ－Ｐｘ活力，降低ＭＤＡ含量。这表明，脑缺血过程中ｃ－ｆｏｓ原 癌基因呈现一过性高表达，Ｂｅｒ的抗氧自由基及降低ｃ－ｆｏｓ ｍＲＮＡ水平的作用可能是其抗脑缺血 作用机制之一。
Dot blotting assay was employed in this study to observe dynamic changes of c-fos mRNA expression during reperfusion after cerebral ischemia in rats, and effect of berberine (BER) on c-fos expres- sion induced by global cerebral ischemia was studied. The results showed that c-fos gene expression was tem- porally increased in global cerebral ischemia by 5.1 times, as compared with sham-operated group. However, the changes of c-fos gene expression could be inhibited dramatically by BER 20 mg/(kg·d) × 5 d ip. The same dosage of BER significantly increased activities of SOD and GSH-Px and reduced content of MDA of brain tissues. The results suggested that the inhibitory actions of BER on c-fos expression induced by global cerebral ischemia may be related to its protective action against brain ischemia.
出处
《中草药》
CAS
CSCD
北大核心
1999年第1期32-34,共3页
Chinese Traditional and Herbal Drugs
基金
江苏省科委资助!BS94021
关键词
小檗碱
脑缺血
原癌基因
C-FOS
氧自由基
berberine cerebral ischemia proto-oncogene c-fos oxygen free radical
