摘要
为明确红树内生细菌CⅢ-1菌株的抗菌蛋白对香蕉炭疽病的控制作用,采用平板对峙法结合显微观察测定其对致病菌的抑制活性,进行抗菌蛋白对香蕉炭疽病的防病试验,并测定了香蕉果实内丙二醛的含量及防御酶活性的变化。结果表明,CⅢ-1抗菌蛋白粗提液喷雾后立即接种香蕉炭疽病菌,处理8d后其防治效果达71.44%,10d后防效仍有56.67%;防病机制测定表明,(1)CⅢ-1抗菌蛋白对香蕉炭疽病菌的菌丝生长及分生孢子萌发等具有极强的抑制作用,处理后的病菌菌丝出现肿胀、膨大、成泡囊状畸形,分生孢子基本不萌发。(2)抗菌蛋白处理香蕉后,与对照比,抗菌蛋白处理的香蕉丙二醛(MDA)的含量较低,同时过氧化物酶(POD)、苯丙氨酸解氨酶(PAL)等活性也均有不同程度的变化。
In order to evaluate the efficacy of the extra cellular antimicrobial proteins （EACP） of CⅢ-1 to anthracnoses in banana, the inhibition effect on the pathogen of Colletotrichum musae were tested using dual culture method and optical microscope observation, plant biocontrol tests were also carried out with EACP and the change in the content of MDA and activity of SOD, POD and PAL were measured as well. The results showed that： the control effects of the ECAP on the banana anthracnoses were 71.44% and 56.67% 8 d and 10 d after application,respectively. This suggested that the anthracnose of banana was controlled effectively with the ECAP. The research on biological control mechanisms showed that ECAP could inhibit the mycelial growth and reduce the germination of Colletotrichum musae. And treatment with ECAP could reduce the contents of MDA, and at same time could induce the variances of the activities of POD and PAL in the banana and capsicum fruits, cormpared with sterile water and PSB treatment.
出处
《果树学报》
CAS
CSCD
北大核心
2010年第4期580-584,共5页
Journal of Fruit Science
基金
广东省科技计划(2009B020310015
2009B030803055)
关键词
香蕉
抗菌蛋白
炭疽病
生物防治
机制
Bananas
Antimicrobial proteins
Anthracnose
Bio-control
Mechanism
