摘要
建立了快速测定鱼肉中氯霉素残留的高效液相色谱-串联质谱方法。采用乙酸乙酯提取样品中的氯霉素残留,浓缩提取物用正己烷脱脂净化后,用反相液相色谱分离,以液相色谱-串联质谱仪测定,外标法定量。该法对氯霉素的线性范围为0.04～1.0μg/kg,相关系数R2=0.9999,在0.10、0.20、0.40μg/kg3个添加水平范围内的回收率为91.0%～100.1%,相对标准偏差为2.6%～4.1%,检测限为0.01μg/kg,定量限为0.02μg/kg。
An High-performance liquid chromatographic method with tandem mass spectrometry （HPLC-MS/MS） was developed for the determination of Chloramphenicol （CAP） residue in fish.The CAP residue was extracted by ethyl acetate.Concentrated extracts were redissolved in 1 mL dissolving solution and defatted by hexane.The solution was separated on a RP-HPLC column and detected by HPLC-MS/MS with positive electrospray ionization（ESI-） in multiple reaction monitoring（MRM） mode.Quantitation was carried out using an external standard method.The standard curve for CAP was linear over the concentration range of 0.04-1.0 tzg/kg with correlation coefficients （R2） of 0.9999. The recoveries of CAP from fish samples spiked with the concentration levels at 0.10, 0.20, 0.40μg/kg were range of 91.0%-100.1%. The relative standard deviations were 2.6%-4.1%. The limit of detection for the method was 0.01 μg/kg. The limit of quantification for the method was 0.02μg/kg. Key words： High performance liquid chromatography-tandem mass speetrometry（HPLC-MS/MS）; Chloramphenicol; Residue; Fish
出处
《广东农业科学》
CAS
CSCD
北大核心
2010年第7期217-219,共3页
Guangdong Agricultural Sciences
基金
江西省自然(青年)科学基金项目(2007GQC1094)
关键词
高效液相色谱-串联质谱法
氯霉素
残留
鱼肉
High performance liquid chromatography-tandem mass spectrometry（HPLC-MS/MS）
Chloramphenieol
Residue
Fish
