摘要
建立了鸡肉中3种氯霉素类抗生素残留的高效液相色谱 电喷雾电离三级四极杆质谱(HPLC ESI MS MS)测定法。该方法采用多反应监测(MRM)负离子模式,可一次对鸡肉中的氯霉素、甲砜霉素和氟甲砜霉素进行定性和定量。该方法仅需1g样品,并省去固相萃取步骤,具有操作简便、有机试剂消耗量少、测定周期短等优点。方法的检出限为0 010μg/kg,测定低限为0 100μg/kg,线性范围为0 050～1 00μg/L,加标回收率为69 0%～92 8%,相对标准偏差为6 3%～12 9%。
The method for the analysis of three chloramphenicols including chloramphenicol, thiamphenicol and florfenicol in chickens was developed by high performance liquid chromatography combined with electrospray ionization mass spectrometry (HPLC-ESI-MS-MS). The mass spectrometer was operated in the negative ion mode using multiple reaction monitoring for qualitative and quantitative analysis of these compounds at the same time. Only 1 g of sample was needed, and no solid phase extraction (SPE) procedure was adopted. The advantages of the method are simple operation, less organic chemicals consumed, and shorter operation time. The limits of detection (LOD) were 0.010(μg/kg), and the limits of quantitation (LOQ) were 0.100 (μg/kg). The linear plots were obtained between 0.050 and 1.00 (μg/L). Overall recoveries were between 69.0%and 92.8%with relative standard deviations between 6.3%and 12.9%.
出处
《色谱》
CAS
CSCD
北大核心
2005年第1期92-95,共4页
Chinese Journal of Chromatography
基金
国家质量监督检验检疫总局科技项目(2003IK047)资助.
关键词
高效液相色谱-电喷雾电离质谱
氯霉素
甲砜霉素
氟甲砜霉素
残留
鸡肉
high performance liquid chromatography-electrospray ionization mass spectrometry
chloramphenicol
thiamphenicol
florfenicol
residue
chicken
