摘要
目的:探讨五味子冻干粉改善睡眠剥夺果蝇睡眠的作用。方法:在恒温、恒湿、自动光控条件下,以7日龄果蝇作为观察对象,分别采用光照和机械刺激两种不同的方法,复制果蝇的睡眠剥夺模型。利用果蝇活动监测仪(DAMS),以静止时间持续5min以上作为睡眠标准,观察以8%五味子冻干粉连续给药6天(雌果蝇)或给药4天(雄果蝇)后,果蝇睡眠时间的变化。结果:两种方法均可剥夺果蝇的睡眠,与正常果蝇相比,睡眠剥夺果蝇的睡眠时间明显缩短(P<0.01)。给予五味子冻干粉后,无论光照刺激还是机械刺激所致睡眠剥夺果蝇的睡眠时间均明显增加,与睡眠剥夺模型对照组比较,具有显著性差异(P<0.01)。结论:8%五味子冻干粉对睡眠剥夺果蝇的睡眠有一定改善作用。
This work aimed to study the effect of Fructus Schisandrae Chinensis Freeze-dried Powder on improving the sleep of sleep-deprived Drosophila melanogaster. Under the standard conditions of constant temperature, humidity, automatically controlled lighting, 7-day-old drosophilae were observed. Their sleep was deprived by illumination or mechanical stimulation to establish sleep deprivation models. An infrared drosophila activity monitor system （DAMS） was used to observe and analyze the recorded data and define drosophilae＇s quiescing time exceeding 5 min as the sleeping time. 8% drug substratum was given to females for 6 days and to males for 7 days. Drosophilae＇s sleeping time was calculated. The sleep-deprived models of drosophilae were established by both methods. Compared with the control group, the sleeping time of sleep-deprived drosophilae was decreased （P〈0.01）. The sleeping time of the drug treatment groups was significantly prolonged no matter which method was used to establish the model （P〈0.01）. The results showed that Fructus Schisandrae Chinensis Freeze-dried Powder at the dosage of 8% may improve the sleep of sleep-deprived models induced by two different methods.
出处
《世界科学技术-中医药现代化》
2010年第3期409-412,共4页
Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology
关键词
五味子
果蝇
睡眠剥夺
Fructus Schisandrae Chinensis, Drosophila melanogaster, Sleep deprivation
