摘要
目的探讨五味子对中毒大鼠肝细胞核基质蛋白和结构的保护作用。方法将Wistar大鼠随机分为对照组、中毒组和治疗组。中毒组和治疗组大鼠经腹腔一次性注射200mLLCCl4(0.2mL100g体质量)致毒。治疗组以五味子提取液[0.5mL(100g·d)]灌胃,中毒组以同量生理盐水灌胃,不同时间处死大鼠,光镜、电镜下观察肝脏细胞结构,并用免疫组化方法检测LaminB蛋白的表达和电泳分析核基质蛋白成分。结果光镜和电镜下,五味子提取物治疗后大鼠肝细胞病理改变明显减轻。五味子提取物促进了中毒大鼠肝细胞LaminB蛋白的表达、并有调节大鼠肝细胞核基质蛋白代谢的作用。结论五味子可能通过促进CCl4中毒大鼠肝细胞LaminB蛋白的表达和影响核基质蛋白的代谢而促进损伤肝细胞修复,对于CCl4中毒大鼠肝细胞核基质蛋白和结构具有保护作用。
Objective To explore the protecting effects of schisandrins on the nuclear matrix proteins and structure of hepatoeyte of CCl4-intoxicated rat. Methods The Wistar rats were randomly divided into the control group, the intoxicated group, and the treating group. The rats of intoxicated and treating group were given one-time peritoneal injection of CCl4 （200 mL/L in vegetable oil） at a dosage of 0.2 mL/ 100 g body mass. The CCl4-intoxicated rats in the treating group were treated with schisandrins extract by oral administration [ 0.5 mL/ （ 100 g·d） ], while saline was given to rats in the intoxicated group. The rats were sacrificed at different time pionts. The morphologic changes of the hepatocytes were examined under the light and electron microscope. The expression of lamin B and the metabolism of nuclear matrix proreins were analyzed by immunohistochemical method and SDS-PAGE, respectively. Results The application of schisandrins extract attenuated the pathological changes of the hepatocyte, increased the expression of lamin B protein, and modulated the metabolism of nuclear matrix proreins. Conclusion Schisandrins may promote eytothesis of damaged hepatocytes by increasing the expression of lamin B protein and modulating the metabolism of nuclear matrix proteins in CCl4-intoxicated rat, thus, schisandrins play a role of protecting the nuclear matrix proteins and the structure of hepatocyte in CCl4-intoxicated rat.
出处
《免疫学杂志》
CAS
CSCD
北大核心
2006年第4期455-459,共5页
Immunological Journal
基金
广东省中医药局(103068)
广东省汕头市重点科技计划项目资助(20031196)
