摘要
在低温需求型植物的发育过程中，春化作用是诱导植物成花的必要因子。在冬小麦春化进程中存在着一个核酸代谢的关键期，为了探讨春化作用的分子机理，以不春化及脱春化的冬小麦京冬1号（Ｔｒｉｔｉｃｕｍ ａｅｓｔｉｖｕｍＬ．ｃｖ．Ｊｉｎｄｏｎｇ Ｎｏ．１）胚芽的ｍＲＮＡ为对照，通过减法杂交构建了富集冬小麦春化相关基因的ｃＤＮＡ文库，经过差异筛选分离到了一个仅在春化２１ｄ这一关键期表达，而在不春化、春化４ｄ、脱春化不表达的春化相关。ｃＤＮＡ克隆Ｖｒｃ７９。Ｎｏｒｔｈｅｍ杂交及同源性分析表明它是一个在植物中新发现的不同于低温胁迫诱导基因的春化特异克隆，其对春化需求型植物的成花诱导可能起重要的调控作用。
Vernalization is an essential factor in the flowering development of cold-required plants. There is akey stage of nucleic acid metabolism in the process of vernalization in winter wheat. To probe into the molecular detendnants of vernalization, a cDNA library presumably enriching vernalization-related genes was prepared by specially expressed mRNAs from winter wheat (Triticum aestivum L. cv. Jingdong No. 1 ) plumules at thekey stage of 21 d vernalization being recovered as cDNAs after subtraction with mRNAs from nonvernalized anddevernalized plumules. One vernalization-related cDNA clone (Vrc ), Vrc79, which was only expressed at thekey stage of 21 d vernalization, but not at other stages of nonvernalization, 4 d vernalization and devernalization, was isolated by differential screening of the library, and shown to be a vernalization-specific clone byNorthem blot. Result of homology search suggested that Vrc79 was a novel gene identified in higher plantwhich was different from the cold-stress-induced genes and might play an important role in the floral induction in vemalization-requiring plants.
关键词
春化作用相关cDNA克隆
减法杂交
差异筛选
冬小麦
Differential screening, Subbetractive hybridization, Vernalization-related cDNA clone, Winter wheat
