摘要
对番茄种子芽期过氧化物酶（POD）同工酶与酯酶（EST）同工酶聚丙烯酰胺凝胶电泳（PAGE）方法进行了初步研究。结果表明，POD同工酶电泳酶样提取液以pH6．5～7.5的0.1．mol/L磷酸缓冲液十0．1％巯基乙醇较适宜；EST同工酶酶样提取液以pH6．0～70的0．1mol／LTris-柠檬酸＋0．1％巯基乙醇或0．1mol／L磷酸缓冲液＋0．1％巯基乙醇较适宜。常用的7.5％分离胶浓度不适宜番茄种子芽期POD和EST同工酶电泳分析，采用11．5％的分离胶能达到理想的分离效果。
Study on PAGE method of POD and EST isozyme in tomato germinating seeds was carriedout, the results indicated that the suitable extraction buffer for POD isozyme is pH6. 5～7- 5 0. 1 mol/Lphosphate buffer+0. 1 % mercaptoethanol, and that for EST isozyme is pH 6. 0～7. 0 0. 1 mol/L Triscltrate +0. 1% mercaptoethanol or 0. 1 mol/L phosphate buffer + 0. 1 mol/L mercaptoethanol. The7. 5% separating gel concentratlon was not suitable for both lsozymes in question, it should be replacedwith 11. 5% concentration in order to achieve satisfactory electrophoresis effect.
出处
《西北农业学报》
CAS
CSCD
1999年第1期87-90,共4页
Acta Agriculturae Boreali-occidentalis Sinica
