摘要
目的 研究在不同病理类型的胶质瘤中1p/19q的联合缺失情况及相关的临床因素.方法 收集胶质瘤标本127例,采用荧光原位杂交技术检测肿瘤组织标本中1p和19q的缺失状态,统计分析不同病理类型胶质瘤组织的1p/19q联合缺失率并应用Logistic回归分析与联合缺失相关的临床因素.结果 1p/19q联合缺失率为42.52%,其中星形细胞肿瘤、少枝星形细胞肿瘤和少枝胶质细胞肿瘤中1p/19q联合缺失率分别为19.30%、50.00%和80.77%,少枝星形细胞肿瘤和少枝胶质细胞肿瘤的1p/19q联合缺失率显著高于星形细胞肿瘤(P〈0.01),少枝胶质细胞肿瘤的1p/19q联合缺失率高于少枝星形细胞肿瘤(P〈0.05);1p/19q单独缺失率为9.45%,星形细胞肿瘤、少枝星形细胞肿瘤和少枝胶质细胞肿瘤的1p/19q单独缺失率分别为12.28%、11.36%和0.1p/19q联合缺失与年龄、性别和病理级别的相关分析无统计学意义.结论 在胶质瘤中,联合缺失是染色体1p和19q最常见的缺失形式,并且与少枝胶质细胞成分密切相关,在胶质瘤的病理诊断、治疗方案选择和预后判断中有一定价值.
Objective To investigate the frequencies of combining loss ofheterozygosity(LOH)of chromosome 1p/19q in gliomas of different pathologies and analyze the clinical factors correlated withcombining LOH by logistic regression.Methods Tumor samples from 127 giioma patients were collected.The status of 1p and 19q was evaluated by fluorescence in situ hybridization(FISH)and the frequencies of combining 1p/19q LOH in gliomas of different pathologies were analyzed.Results The frequencies of combining 1p/19q LOH in agtrocytic,oligoagtrocytic and oligodendrnglial tumors were 19.30%,50.00%and 80.77%respectively.The frequencies of combining 1p/19q LOH in oligoagtrocytic and oligodendroglial tumors were hisher than those in agtrocytic tumors(P〈0.01)and the frequencies of combining 1p/19q LOH in oligedendroglial tumors was higher than those in oligoagtrocytic tumors(P〈0.05).The frequencies of 1p/19q LOH in agtrocytic,oligoagtrocytic and oligodendroglial tumors were 12.28%,11.36 and 0 respectively.There Wag no significant correlation between combining 1p/19q LOH and age,gender and grade by logistic regression.Conclusion In gliomas,combining 1p/19q LOH is proved to be the most common pattern of chromosome deletion involving 1p or 19q.It is significantly correlated with oligedendrogiial component.Combining 1p/19q LOH may be valuable in the diagnosis,treatment and prognostic prediction for glioma with oligodendroglial component.
作者
任晓辉
崔向丽
林松
王忠诚
REN Xiao-hui;CUI Xiang-li;LIN Song;WANG Zhong-cheng(Beijing Neurosurgical Institute,Beijing 100050,China)
出处
《中华医学杂志》
CAS
CSCD
北大核心
2010年第25期1781-1784,共4页
National Medical Journal of China
基金
国家“973”计划专项课题(2007CB512503)
