摘要
目的探讨运动对慢性应激大鼠不同脑区磷酸化细胞外信号调节蛋白激酶(p-ERK)-环磷酸腺苷反应元件结合蛋白(p-CREB)信号通路的作用。方法雄性SD大鼠40只随机分为对照组、应激组、运动+应激组及运动组;采用慢性束缚方式建立应激模型;运动方式为无负重游泳;免疫组织化学方法检测海马、额叶皮层、纹状体p-ERK/p-CREB表达变化。结果应激组海马、额叶皮层、纹状体区p-ERK表达分别为(11.25±3.68),(19.26±6.03),(18.22±4.70),p-CREB表达分别为(2.13±0.38),(2.32±0.49),(2.91±0.54),对照组各脑区p-ERK表达分别为(22.53±2.96),(30.64±5.89),(29.46±4.54),p-CREB表达分别为(3.32±0.33),(3.56±0.44),(3.94±0.51),应激组表达低于对照组,差异有统计学意义(P<0.01);运动+应激组海马、额叶皮层p-ERK表达为(17.59±4.30),(25.31±5.57),p-CREB表达为(2.76±0.40),(2.98±0.53),表达高于应激组,差异有统计学意义(P<0.05)。结论运动锻炼作为一种有效的防护和干预手段,可能通过上调ERK-CREB通路提高慢性应激状态下的学习记忆能力。
Objective To probe into the effect of exercise on phosphorylated extracellular signal-regulated protein kinase（p-ERK） and phosphorylated cAMP response element binding protein（p-CREB） pathway in different cerebral areas in rats with chronic stress.Methods Forty male SD rats were randomly divided into 4 groups：control,stress,exercise stress and exercise group.The rat model of chronic stress was made by restraint and the exercise mode by unloading swimming.pERK and p-CREB in the frontal cortex,hippocampus and striatum were examined with immunohistochemistry assays.Results Compared with the control group,p-ERK and p-CREB expression in the frontal cortex,hippocampus and striatum were significantly decreased in the stress group（P 0.01）.Compared with stress group,exercise stress group showed higher levels of p-ERK and p-CREB with statistical significance in frontal cortex and hippocampus（P 0.05）.Conclusion As an effective protection measure,long-term exercise may enhance learning and memory in condition of chronic stress by upregulating ERK-CREB pathway.
出处
《中国公共卫生》
CAS
CSCD
北大核心
2010年第7期848-849,共2页
Chinese Journal of Public Health
基金
四川省科技厅青年基金(09ZQ026-078)
