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组蛋白去乙酰化酶抑制剂对人骨肉瘤细胞抑制增殖和诱导分化的研究 被引量：2

Histone deacetylase inhibitor in inhibiting proliferation and inducing differentiation of human osteogenic sarcoma cells
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摘要 [目的]观察组蛋白去乙酰化酶抑制剂曲古抑菌素A(TSA)对人骨肉瘤MG-63细胞增殖和分化的影响。[方法]不同浓度的TSA作用MG-63细胞,采用噻唑蓝(MTT)法、倒置相差显微镜观察药物对细胞生长的影响并绘制细胞生长曲线;软琼脂集落形成实验观察细胞生长和侵袭能力的变化;流式细胞仪测定细胞周期变化。[结果]TSA可明显抑制MG-63细胞增殖,并呈现剂量和时间的依赖性;细胞形态呈明显的良性分化;瘤细胞的软琼脂集落形成率明显降低;TSA能够延缓细胞周期G1-S进程,阻滞细胞于G1期,停留在G2/M期。[结论]TSA对MG-63细胞有明显的抑制增殖和诱导分化作用。 [ Objective] To study the effect of histone deacetylase inhibitor- Trichostatin A （TSA） on proliferation and differentiation of human osteogenic sarcoma cell. [ Methods] Human osteogenic sarcoma cell line MG-63 was treated in vitro with various concentrations of TSA, a potent and specific histone deacetylase inhibitor. Proliferation suppression was observed by MTT method and inverted microcopy before and after TSA treatment, and the cell growth curve was obtained. The cell growth and invasion ability were measured with the colony -formation rate in soft agar test. Flow cytometry was used to investigate the cell cycle. [ Results ] TSA significantly inhibited the proliferation of the human osteogenic sarcoma cell in a dose - dependent and time - dependent manner. The cell experienced benigh morphological differentiation. The colony - formation rate in semi - solid agar was significantly decreased （P 〈0. 01 ） . TSA delayed cell cycle G1 - S progression and induced a G1 cell cycle arrest. TSA can arrest the cells of cultured MG - 63 cells at G2/M phase. [ Conclusion] TSA can inhibit the proliferation of osteogenic sarcoma cell and lead to benign differentiation.
作者 丁凡 邵增务
机构地区 华中科技大学同济医学院附属协和医院骨科
出处 《中国矫形外科杂志》 CAS CSCD 北大核心 2010年第13期1111-1114,共4页 Orthopedic Journal of China
关键词 骨肉瘤 组蛋白去乙酰化酶 曲古抑菌素A 分化 osteosarcoma, histone deacetylase, trichostain A, differentiation
分类号 R738.1 [医药卫生—肿瘤]
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参考文献13

  • 1Lachner M, OCmToll D, Rea S,et al. Methylation of histoneH3 lysine9 creates a binding site for HPI proteins[J]. Nature,2001, 410:116 - 120.
  • 2Tom H, Peggy P, Greetje E,et al. Trichostatin A, a critical factor in maintaining the functional differentiation of primary cultured rat hepa- tocytes[J]. Toxicol App Pharmaco1,2007,218 : 64 - 71.
  • 3Jyothsna R, Dipanjan B, Bidisha B, et al. Trichostatin - A induces differential changes in histone protein dynamics and expression in HeLa cells [ J ]. Biochem Biophys Res Commun,2007,363:263 - 268.
  • 4尚建强,贾婷,陈方方,张嵩,姜淑娟.去甲基化和组蛋白去乙酰化转移酶抑制剂对Ishikawa和AN3细胞增殖和凋亡的影响[J].中华肿瘤防治杂志,2007,14(9):666-669. 被引量：1
  • 5Nicole S, Sabine S, Careen K,et al. The histone deacetylase inhibitor trichostatin A mediates upregulation of 5 - lipoxygenase promoter activity by recruitment of Spl to distinct GC -boxes[ J]. Biochem Biophys Acta,2007,1771 : 1271 -1282.
  • 6Mohsen H, Koji H, Koh O, et al. Trichostatin A induces myocardial differentiation of monkey ES cells [ J ]. Biochem Biophys Res Commun,2007,356 : 386 -391.
  • 7Jing - Ni O, Je rome T, Alexander U, et al. Histone deacetylase inhibitor trichostatin A induces global and gene - specific DNA demethylation in human cancer cell lines [ J ]. Biochem Pharmacol,2007,73 : 1297 - 1307.
  • 8Piotrowska H, Jagodzinski PP. Trichostatin A, sodium butyrate, and 5 - aza - 2 - deoxycytidiue alter the expression of glucocorticoid receptor a and b isoforms in Hut - 78 T - and Raji B - lymphoma cell lines [ J]. Biomed Phannacother,2007, 61 : 451 -454.
  • 9Eunjin K, Russell B, Timothy C,et al. Trichostatin A and 5 -aza -2 - deoxycytidine switch S1P from an inhibitor to a stimulator of motility through epigenetic regulation of SIP receptors[ J]. Cancer Lett, 2007,250:53 - 62.
  • 10Tom H, Peggy P, Greetje E ,et al. Trichostatin A, a critical factor in maintaining the functional differentiation of primary cultured rat hepatocytes [ J ]. Toxicol Appl Pharmacol,2007,218 : 64 - 71.

二级参考文献18

  • 1Li Hua Liu1,Wen Hua Xiao2,Wei Wen Liu3 1Department of Oncology, Southwest Hospital, Third Military Medical University, Chongqing 400038, China (now working in Department of Gastroenterology, General Hospital of PLA, Lanzhou 730050, Gansu Province, China)2Department of Oncology3Department of Gastroenterology, Southwest Hospital, Third Military Medical University, Chongqing 400038, China.Effect of 5-Aza-2'-deoxycytidine on the P16 tumor suppressor gene in hepatocellular carcinoma cell line HepG2[J].World Journal of Gastroenterology,2001,7(1):131-135. 被引量：21
  • 2解先宽,杨迪生,叶招明,陶惠民.反义c-myc重组腺病毒的构建及其抗骨肉瘤细胞的作用[J].癌症,2005,24(3):292-297. 被引量：24
  • 3GREWAI S I, MOAZED D. Heterochromatin and epigenetic control of gene expression[J]. Science, 2003, 301(5634):798-802.
  • 4IKURA T, OGRYZKO V V, GRIGORIEV M, et al. Involvement of the TIP60 histone acetylase complex in DNA repair and apoptosis [ J ]. Cell, 2000, 102 (4) : 463-473.
  • 5ELIZABETH L, DUNPHY T J, et al. Requirement for TAFII250 acetyltransferase activity in cell cycle progression[J]. Mol Cell Biol, 2000, 20: 1134-1139.
  • 6BALLESTAR E, WOLFFE A P. Methyl-CpG2binding proteins:Targeting specific gene repression[J]. Eur J Biochem, 2001,268 (1): 1-6.
  • 7PIVOT-PAJOT C, CARON C, GOVIN J, et al. Acetylation-dependent chromatin reorganization by BRDT, a testis-specific bromodomain-containing protein[J]. Mol Cell Biol, 2003, 23 ( 15 ) : 5354-5365.
  • 8SCHROEDER T M, WfESTENDOR J J. Histone deacetylase inhibitors promote osteoblast maturation[J]. J Bone Miner Res, 2005, 20( 12): 2254-2263.
  • 9WU P, MENG L, WANG H, et al. Role of hTERT in apoptosis of cervical cancer induced by histone deacetylase inhibitor[J ].Bioehem Biophys Res Commun, 2005, 335 ( 1 ) : 36-44.
  • 10PEITONEN K, KVIGAR.IUL T M, JARVINEN P M, et al. Melanoma cell lines are susceptible to histonc deacetylase inhibitor TSA provoked cell cycle arrest and apoptosis [ J ].Pigment Cell Res, 2005, 18(3): 196-202.

共引文献10

同被引文献78

  • 1钱其军,张琪.肿瘤靶向治疗的现状和展望[J].中国肿瘤生物治疗杂志,2006,13(4):239-242. 被引量：7
  • 2陈运贤,朱小玉.肿瘤治疗新目标——肿瘤干细胞[J].中国肿瘤,2007,16(7):518-520. 被引量：3
  • 3Conti CM, Angelucci D, Ferri M, Maccauro G, Caraffa A, Doyle R, et al. Relationship between cancer and psychology: An updated history. J Biol Regul Homeost Agents 2011; 25(3): 331-9.
  • 4Ciurea ME, Georgescu AM, Purcaru SO, Artene SA, Emami GH, Boldeanu MV, et al. Cancer stem cells: Biological functions and therapeutically targeting. Int J Mol Sci 2014; 15(5): 8169-85.
  • 5Reya T, Morrison S J, Clarke MF, Weissman IL. Stem cells, cancer, and cancer stem cells. Nature 2001; 414(6859): 105-11.
  • 6Bu Y, Cao D. The origin of cancer stem cells. Front Biosci (Schol Ed) 2012; 4: 819-30.
  • 7Renan MJ. How many mutations are required for tumorigenesis? Implications from human cancer data. Mol Carcinog 1993; 7(3): 139-46.
  • 8Takao S, Ding Q, Matsubara S. Pancreatic cancer stem cells: Regulatory networks in the tumor microenvironment and targeted therapy. J Hepatobiliary Pancreat Sci 2012; 19(6): 614-20.
  • 9Cheng CY, Hwang CI, Corney DC, Flesken-Nikitin A, Jiang LC, Oner GM, et al. miR-34 cooperates with p53 in suppression of prostate cancer by joint regulation of stem cell compartment. Cell Reports 2014; 6(6): 1000-7.
  • 10Si ML, Zhu S, Wu H, Lu Z, Wu F, Mo YY. miR-21-mediated tumor growth. Oncogene 2007; 26(19): 2799-803.

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二级引证文献7

中国矫形外科杂志
2010年 第13期

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