摘要
目的研究组蛋白去乙酰化酶抑制荆对乳腺癌细胞株增殖周期的影响。方法培养乳腺癌细胞株MCF-7，用TSA、SAHA、CS055、MS-2754种不同类型的组蛋白去乙酰化酶抑制剂分别作用于细胞，于24、48、72、96h用MTr比色法观察细胞生长情况，初筛出高效的抑制剂。用所选出的抑制剂的不同浓度处理细胞，流式细胞术检测S期细胞的比例和周期素CyclinD1、CyclinA2，并进行统计分析。结果在TSA、SAHA、CS055和MS-2754种不同类型的组蛋白去乙酰化酶抑制剂中，SAHA显示出较强的抑制能力（P〈0．05）；在一定时间和浓度范围内，其抑制能力有明显的时效关系；在抑制剂作用下，S期细胞比例和周期素CyelinA2表达下降，CyelinD1表达上升（P均〈0．05）。结论组蛋白去乙酰化酶抑制剂SAHA是乳腺癌细胞增殖的高效抑制剂，其抑制作用有一定的时效关系；周期素CyclinD1、CyelinA2参与了抑制剂对细胞增殖周期的调控。
Objective To study the effect of histone deacetylase inhibitors on cell cycle of breast cancer cell line MCF-7. Methods The breast cancer cell line MCF-7 was treated by histone deacetylase inhibitors （TSA, SAHA,CS055,MS-275） respectively and observed by MT＇F assay after 24 hours, 48 hours, 72 hours, 96 hours. The chosen inhibitor was used to treat MCF-7 cell in different concentration. The flow cytometry was used to detect the S- phase cell and Cyclin A2, Cyclin D1. SPSS 10.0 was used to analyze the data. Results Within the four inhibitors, SAHA showed the must powerful effect of depression of cell growth and time-effect relation. The percentage of S-phase ceils and level of Cyclin A2 decreased, the level of Cyclin D1 increased. Conclusions SAHA is the most powerful histone deacetylase inhibitors for breast cancer cell MCF-7 , the effect of depression of cell growth shows time-effect relation. Cyclin A2 and Cyelin D1 were involed in this regulation of cell cycle.
出处
《中国综合临床》
2009年第12期1238-1240,共3页
Clinical Medicine of China
基金
云南省科技计划项目（2007C0026R）
