摘要
目的:建立梯度洗脱测定肾康灵颗粒中丹参素、原儿茶醛含量的HPLC法。方法:Hypersil ODSCl8柱,流动相甲醇-1%冰醋酸溶液,线性梯度洗脱:0～8 min(5∶95),8～11 min(6∶94～5∶95),11～20 min(5∶95);流速1 mL.min-1;检测波长280 nm。结果:在此色谱条件下2种成分可完全分离。丹参素、原儿茶醛的线性范围分别为0.050 4～0.504μg(r=0.999 8),0.109 0～1.090μg(r=0.999 9)。丹参素、原儿茶醛在肾康灵颗粒中的平均回收率分别为98.40%,98.85%,RSD 1.2%,0.87%。结论:该方法简便、准确、快速,可同时测定肾康灵颗粒中丹参素、原儿茶醛的含量。
Objective： To develop an HPLC method for gradient elution determination of danshensu and protocatechuic aldehyde in Shengkangling granula. Method： The analysis was carried on a column of Hypersil ODS2-Cls with a mobile phase consisted of methanol-1% glacial acetic acid 0-8 min（5： 95）, 8-11 min（6： 9±5：95）, 11-20 min（5： 95） gradient elution at the flow rate of 1 mL·min^-1. The detection wavelength was 280 nm. Result： The linearity was obtained in the range of 0. 050±0. 504μg（ r = 0. 999 8 ） for tanshinol and 0. 109 0-1. 090 μg（ r = 0. 999 9）for protocatechuic aldehyde, respectively. The average recoveries of tanshinol and protocatechuic aldehyde in Shenkangling granula were 98.40% and 98.85% , and the RSDs were all less than 2.0%. Conclusion： The method is simple, accurate and rapid. It may be suitable for the usual quality control of tanshinol and protocatechuic aldehyde in Shengkangling granula.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2010年第13期1699-1701,共3页
China Journal of Chinese Materia Medica
基金
福建省自然科学基金课题(2008J0093)
