摘要
目的研究转化生长因子(TGF)-β1对肝星状细胞(HSC)中胰岛素样生长因子结合蛋白相关蛋白1(IGFBPrP1)及胶原纤维表达的影响及意义。方法①将TGF-β1作用于体外培养的HSC-T624h,采用Westernblot法检测HSC-T6胞质蛋白中IGFBPrP1和培养上清液中Ⅰ型胶原的表达,并进行IGFBPrP1与Ⅰ型胶原的相关性分析;②应用IGFBPrP1处理HSC-T648h,采用Westernblot法检测上清中Ⅰ型胶原的表达。结果①与正常对照组相比,TGF-β1处理组IGFBPrP1和Ⅰ型胶原的表达明显增加(P<0.05),且IGFBPrP1的表达与Ⅰ型胶原的表达呈正相关(P<0.05);②IGFBPrP1处理组Ⅰ型胶原的表达较正常对照组明显增高(P<0.01)。结论 TGF-β1可引起HSC-T6过表达IGFBPrP1;IGFBPrP1可使HSC-T6胶原纤维含量增多。表明IGFBPrP1具有促肝纤维化的作用。
Objective To investigate the effects of transforming growth factor -β1 （TGF-β1） on expression of IGFBPrP1 and collagen I in hepatic stellate cells （HSC-T6）. Methods ①Twenty-four hours after cultured HSC-T6 cells were treated by TGF-β1, Western blotting was used to determine the expression levels of IGFBPrP1 in cytoplasm and of collagen I in culture supernatant. Correlation between the two was analyzed. ②Forty-eight hours after HSC-T6 cells were treated by IGFBPrP1, the expression of collagen I in the culture supernatant was determined by Western blotting. Results ①The expression levels of IGFBPrP1 and collagen I in TGF-β1 treated cells were significantly high- er than those in normal controls （P0.05）. There was a significantly positive correlation between IGFBPrP1 and colla-gen I in HSC-T6 treated TGF-β1 （P0.05）. ②The expression of collagen I in IGFBPrP1-treated HSC-T6 cells was significantly increased as compared with normal controls （P0.01）. Conclusion TGF-β1 was shown to cause an over- expression of IGFBPrP1, which in turn may result in increased production of collagen I in HSC-T6 cells, suggesting that IGFBPrP1 promotes the process of liver fibrosis.
出处
《中国药物与临床》
CAS
2010年第7期729-731,共3页
Chinese Remedies & Clinics
基金
国家自然科学基金(30871146)
山西省回国留学人员科研资助项目(2009-44)
关键词
胰岛素样生长因子结合蛋白质1
转化生长因子Β
肝硬化
Insulin-like growth factor binding protein1
Transforming growth factar beta
Liver cirrhosis
