摘要
目的比较不同培养条件下原代人胸腺上皮细胞生长情况，观察上清液对T细胞林85细胞增殖及存活的效应。方法原代胸腺上皮细胞培养、85细胞增殖测定、台盼兰试验及FACS分析。结果合血清的培养基与合生长日子的培养基均能培养出原代胸球上皮细胞，TES上清单独能促使85细胞增殖且能提高85细胞的存活率。结论研究发现合血清的培养基与合生长因子的培养基培养效果无明显差异，仅剪切也能培养纯净的麻腺上皮细胞。TES上清单独能促使85细胞增殖，提示上清液中可能存在一种未知的细胞因子。上清液能维持85细胞生存，可能与影响T细胞生长周期有关。
Objective Primary human thymic epithelial cell growth was compared under different culture conditions.The effect of supernatant on T Cell line 85 cell proliferation and on 85 cell srvival were investigated. Methods Culthe ofhuman thymic epithelial cells .Proliferation assay of 85 Cells, Trypan- blue staining and FACS analysis . Results Serum containing medium and growth factor containing medium both could culture primary thymic epithelial cells. TES. alone could promote the proliferation of 85 cells and improve the nsurvival rate of 85 cells. Conclusion Ther was no obvious thebetween containing medium and growth factor containing medium. Cutting alone could culture pure thymic epithelialcell. TES could promote the proliferation of 85 Cells. This indicate that there are maybe unknown factors in supernatants. TEScould maitin 85 cell's subsistence. Maybe supernatant affect growth cycle of T cell line.
出处
《广东医学》
CAS
CSCD
1999年第1期6-7,共2页
Guangdong Medical Journal
关键词
胸腺上皮细胞
上清液
细胞增殖
85细胞
Human thymic epithelial cell Supernatant Proliferation Subsistence
