摘要
Supernatants from human primary thymic epithelial cell culture were collected. The proliferationpromoting effect of the supernatant on mouse thymocyte, as well as on Tcell line 85, Be 13, HDMar, Peer, Loucy, Molt4, Reh and Jurkat, were observed. The results demonstrated that the supernatants could increase spontaneous 3 HTdR intake of mouse thymocyte, promote ConAinduced thymocyte proliferation and stimulate the proliferation of 85 cell or the cells of HDMar, Loucy and Jurkat at stationary phase, but did not display any effect on Be13, Peer, Molt4 and Reh cells.
胸腺是细胞分化发育的场所，源于骨髓的前Ｔ细胞。在以胸腺上皮细胞为主体的基质细胞作用下，增殖、分化、成为成熟Ｔ细胞，迁移至外周。胸腺基质细胞通过细胞与细胞之间的直接接触和分泌细胞因子影响细胞分化发育的各个阶段。本文探讨了人胸腺上皮细胞培养上清液（ＴＥＣＳＮ）对鼠胸腺细胞、８５、Ｂｅ１３、ＨＤＭａｒ、Ｍｏｌｔ４、Ｒｅｈ以及Ｊｕｒｋａｔ细胞株增殖的影响。取人胸腺组织，用０．１５％的胰酶消化组织块后，将其接种到培养瓶中进行培养，收集上清液。将ＴＥＣＳＮ加到鼠胸腺细胞悬液中，２ｈ后加３ＨＴｄＲ，观察鼠胸腺细胞自发性掺入３ＨＴｄＲ有无改变。同时将ＴＥＣＳＮ＋ＣｏｎＡ加入鼠胸腺细胞悬液中培养４８ｈ，收获细胞前１８ｈ加入３ＨＴｄＲ，测定ＣＰＭ值。此外，ＴＥＣＳＮ加入８５细胞株及其它细胞株悬液中，观察ＴＥＣＳＮ对这些细胞株增殖的影响。上述各实验均设有对照组（Ｔａｂ．１）。结果表明：ＴＥＣＳＮ本身能增强鼠胸腺细胞３ＨＴｄＲ自发掺入，其３ＨＴｄＲＣＰＭ值明显大于对照组（Ｆｉｇ．１）。ＴＥＣＳＮ单独使用不影响胸腺细胞增殖，ＣｏｎＡ单独作用能促进鼠胸腺细胞增殖反应，而ＴＥＣＳＮ与ＣｏｎＡ共同使用，胸腺?
