摘要
为了分析胸腺细胞在胸腺上皮细胞发育中的作用，本文作者在体外分析了胸腺细胞对小鼠胸腺上皮细胞系（ＭＴＥＣ１）增殖的作用。结果表明，不同的胸腺细胞亚群以及胸腺细胞裂解液均能促进ＭＴＥＣ１增殖，考的松耐受性胸腺细胞和ＣＤ３－４－８－细胞具有同样的促进ＭＴＥＣ１增殖的作用，其促进程度与两种细胞的数量有关，胸腺细胞和ＭＴＥＣ１为２０１或４０１时，其促进作用最强。胸腺细胞裂解液在高浓度（１２稀释）时，能促进ＭＴＥＣ１增殖，在低浓度时则不影响ＭＴＥＣ１的增殖。本实验提示，胸腺细胞可以通过细胞－细胞相互作用以及分泌细胞因子影响ＭＴＥＣ１的增殖。
To analyze the effect of thymocytes on thymic epithelial cell development,we examined the effect of fresh thymocytes on an established mouse thymic epithelial cell line(MTEC1) proliferation in vitro.Different thymocyte populations and thymocyte lysate could significantly increase MTEC1 proliferation.The effect of cortisone resistant thymocytes on MTEC1 proliferation was the same as CD3 -4 -8 - thymocytes and the effect was of a cell dose dependent fashion.The maximal effect was observed when the proportion of thymocytes to MTEC1 was 20∶1 or 40∶1.Relatively high doses of thymocytes lysate could promote MTEC1 proliferation and low doses of it did not affect MTEC1 proliferation.The bulk of the data presented herein suggests that thymocyte may promote MTEC1 proliferation by cell cell interaction and producing cytokine.
出处
《中华微生物学和免疫学杂志》
CAS
CSCD
北大核心
1997年第4期276-279,共4页
Chinese Journal of Microbiology and Immunology
基金
国家自然科学基金
关键词
胸腺上皮细胞
胸腺细胞
Thymic epithelial cell\ \ Thymocyte
