摘要
为研究多器官功能不全综合征（ＭＯＤＳ）时肠道细菌微生态的变化以及Ｇ－杆菌消长与肠道和血液中内毒素水平的关系，本实验选用ＳＤ大鼠，应用无菌Ｚｙｍｏｓａｎ腹腔注射制备大鼠ＭＯＤＳ模型，并对ＭＯＤＳ大鼠肠道菌群进行定量分析，对门静脉和外周静脉血中的内毒素以及肠道游离内毒素含量进行定量测定。结果发现，ＭＯＤＳ状态下肠道细菌微生态发生明显变化，表现为肠杆菌和肠球菌等肠道内需氧菌的数量明显增多，双歧杆菌和乳酸杆菌等肠道专性厌氧菌的数量明显减少，革兰氏阴性肠杆菌（ＧＮＢ）和双歧杆菌的比例倒置；随着肠道菌群变化，ＧＮＢ增多，肠腔内游离内毒素含量增加，肠道内毒素池扩大，而导致门静脉和外周静脉血中内毒素含量增高。从而表明，ＭＯＤＳ时肠道细菌微生态发生明显改变；
To investigate changes of intestinal microecology and its relationships with contents of endotoxin in the portal and peripheral blood as well as the intestinal tract, the MODS model was prepared by intraperitoneal injection of Zymosan A, and the intestinal microflora was analysed quantitatively, the contents of endotoxin in the portal and peripheral blood as well as the intestinal tract were measured. The results showed: 1) there were significant changes in intestinal microflora in the state of MODS, the number of aerobic enterobacteriaceae and enterococci were increased, the number of obligate anaerobic Bifidobacteria and Lactobacillus decreased markedly; 2) with the changing of the intestinal microflora, the number of gram negtive bacteria (GNB) incrased, the endotoxin pool expanded and the levels of endotoxin in peripheral and portal blood increased. It was suggested that there were disturbance of intestinal microecology in the MODS developing process, and proliferation of GNB, intestinal free endotoxin and endotoxemia during MODS was in association with the disturbance of intestinal microecology.
出处
《中国微生态学杂志》
CAS
CSCD
1999年第1期22-24,44,共4页
Chinese Journal of Microecology
关键词
肠道菌群
微生态学
内毒素血症
MODS
Intestinal microflora\ Intestinal free endotoxin\ Endotoxemia\ Multiple organ dysfuntion syndrome
