摘要
目的研究浸润性子宫颈癌(infiltrative cervical cancer,ICC)及子宫颈上皮内瘤变(intraepithelial neoplasia,CIN)中人乳头瘤病毒(human papilom avirus,HPV)感染率及型别分布。方法选取重庆市肿瘤医院组织病理学诊断为CINⅡ、CINⅢ级和宫颈鳞癌的组织标本58例作为研究对象,SPF10LiPA25及HPV DNA酶免疫检测法(DNA enzyme immunoassay,DEIA)进行DNA的提取及扩增;采用线形探针反向杂交技术检测病变组织中HPV型别。结果 58例标本中有56例检测出HPV感染,HPV感染率96.55%,其中CINⅡ、CINⅢ级和子宫颈癌中HPV感染的阳性率分别为100.00%、100.00%、95.00%。共检测出13种HPV型别,HPV16感染率最高(60.00%),其次为HPV18(7.69%)、HPV58(7.69%)和HPV31(4.62%)。宫颈鳞癌、CINⅢ、CINⅡ级HPV多重感染率分别为6.06%、26.31%、33.33%。41～50岁高危型HPV感染率最高(44.64%)。结论 HPV16为重庆市最常见的HPV感染型别,HPV18、HPV58和HPV31也有较高感染率。
Objective To investigate the infection and distribution of human papilloma virus （HPV） types in patients with infiltrative cervical cancer （ICC） and cervical intraepithelial neoplasia （CIN） in Chongqing. Methods Fifty-eight cases diagnosed with ICC or CIN[KG-＊6]Ⅱ to Ⅲ,which were confirmed by histopathology in the Tumor Hospital of Chongqing,were included in the analysis. DNA enzyme immunoassay （DEIA） and SPF10 LiPA25 （version 1） testing was used for DNA extraction and extension,and linear probe reverse hybridization to detect lesions HPV type. Results HPV infection were detected in 56 of 58 specimen,the positive rates of CINⅡ,CINⅢ and cervical cancer were 100.00%,100.00% and 95.00% respectively. A total of 13 types were detected. HPV16 infection rate was up to 60.00%,followed by HPV18（7.69%）,HPV58（7.69%）,and HPV31（4.62%）. Conclusion HPV16 is still the most common type in Chongqing. HPV18,HPV58 and HPV31 also has a high prevalence.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2010年第12期1340-1342,共3页
Journal of Third Military Medical University
基金
中国癌症基金会子宫颈癌专项基金(2007)~~
