摘要
目的观察大鼠脑出血致多器官功能障碍综合征（MODS）模型血清内毒素与TNF-αmRNA基因表达的变化规律及相关性,探讨脑源性多器官功能障碍综合征（CMODS）的可能发生机制。方法将54只Wistar大鼠按随机化原则分为9组：正常对照组6只;假手术组6只;脑出血组42只,分为4、8、12、24、36、48和72h时相点的7个亚组,每亚组6只。向大鼠尾状核内注射Ⅶ型胶原酶0.8U加上适量肝素建立脑出血模型;分别采用偶氮显色法鲎试验定量测定血清内毒素和原位杂交技术测定肺、肝、小肠和肾组织TNF-αmRNA水平;使用CMIA真彩色医学图像分析系统,检测TNF-αmRNA的相对含量。结果脑出血组血清内毒素含量较正常对照组、假手术组明显升高（P〈0.01,P〈0.001）,血清内毒素于术后8h开始升高,24~36h达高峰,72h仍维持较高水平。脑出血组肺、肝、小肠和肾组织TNF-αmRNA的表达自8h开始升高,24~36h达高峰,12~48h各器官组织与正常组和假手术组比较,差异均有统计学意义（P均〈0.01）;肺、肝、小肠和肾组织中TNF-αmRNA的表达与血清内毒素均存在显著相关性。结论大鼠脑出血致MODS模型存在内毒素血症和炎症因子TNF-α在各脏器的异常表达,提示内毒素血症刺激炎症因子TNF-α的异常释放,诱发全身炎症反应,导致MODS的发生。
Objective To examine the relationship between gene expression of TNF-α mRNA and serum endotoxin of the rats following cerebral hemorrhage complicated by multiple organ dysfunction syndrome（CMODS）and to investigate the pathogenesis of CMODS.Methods 54 Wistar rats were randomly divided into nine groups：the normal control group（n=6）,the sham-operative group（n=6）,and the model group was induced in rats by injecting 0.8U collagenase into the caudase putamen（n=42）.The content of endotoxin in the plasma was evaluated with a test box.Gene expression of TNF-α mRNA was assayed using situ hybridization.The area density and the optical density of positive staining expressing TNF-α mRNA were analyzed for the relative content of TNF-α mRNA using situ hybridization and the CMIA medical imaging analysis system.Results There was endotoxemia administration after acute cerebral hemorrhage.It started to increase at 8h after hemorrhage and peaked at 24～36h,and still maintained a high level at 72 h.Expression of TNF-α mRNA in lung,liver,intestines and kidney tissues started to increase at 8h in the model groups and reached a peak at 24～36h.Compared with the normal control and sham-operative groups,expression of TNF-α mRNA was significantly higher at 12～48h in the model groups（P0.01）.Relative analysis indicated that there was significant positive correlation between the TNF-α mRNA expression in lung,liver,kidney,intestine and plasma endotoxin level（P0.01）.Conclusion Endotoxemia occurs after cerebral hemorrhage and gene expression of TNF-α increases,which implies that endotoxemia irritates the release of the inflammatory factor,and systemic inflammatory response syndrome（SIRS）develops.This is the key process in generation of CMODS.
出处
《脑与神经疾病杂志》
2010年第2期121-124,共4页
Journal of Brain and Nervous Diseases
