摘要
目的:探讨丙戊酸钠(VPA)对体外培养的人喉癌Hep-2细胞增殖及环氧化酶2(COX-2)和Survivin mR-NA表达的影响。方法:以1、2、3、4和5mmol/L的VPA处理人喉癌Hep-2细胞12、24、36、48、60和72h后,采用MTT法检测细胞的增殖活性,RT-PCR方法检测3mmoL/LVPA处理Hep-2细胞0、24、48和72h后COX-2和Sur-vivin mRNA的表达。结果:VPA对人喉癌Hep-2细胞的生长具有明显的抑制作用,其作用表现为剂量依赖性(F分别为50.518,80.602,164.699,236.579和243.972,P均<0.001)和时间依赖性(F分别为63.067,126.190,197.807,254.084,294.646和334.773,P均<0.001)。以3mmoL/L的VPA处理人喉癌Hep-2细胞,随作用时间的延长,COX-2和Survivin mRNA的表达逐渐降低(F分别为210.290和76.773,P均<0.001)。结论:VPA对Hep-2细胞具有明显的增殖抑制作用,其作用机制与下调COX-2和Survivin mRNA的表达有关。
Aim：To observe the effects of sodium valproate（VPA） on proliferation and expressions of COX-2 and Survivin mRNA in human laryngeal carcinoma Hep-2 cells.Methods：MTT was used to observe the proliferation of Hep-2 cells treated with various concentrations （1,2,3,4,and 5 mmol/L） of VPA for different time（12,24,36,48,60,and 72 h）. RT-PCR was used to measure the expressions of COX-2 and Survivin mRNA in the Hep-2 cells treated with VPA at 3 mmol/L for different time（0,24,48,and 72 h）.Results：VPA inhibited growth of Hep-2 cells in a dose-dependent manner（F was 50.518,80.602,164.699,236.579,and 243.972,P0.001） and time-dependent manner（F was 63.067,126.190,197.807,254.084,294.646,and 334.773,P0.001）.The expression of COX-2 and Survivin mRNA in the Hep-2 cells treated by VPA at 3 mmol/L decreased（F=210.290 and 76.773,P0.001）.Conclusion：VPA has obvious growth inhibition effect on Hep-2 cells,and the mechanism is related to decrease the expression of COX-2 and Survivin.
出处
《郑州大学学报（医学版）》
CAS
北大核心
2010年第3期440-443,共4页
Journal of Zhengzhou University(Medical Sciences)
