摘要
目的:检测食管鳞状细胞癌组织中哺乳动物雷帕霉素靶蛋白(mTOR)的活性及雷帕霉素(rapa)对食管鳞状细胞癌细胞系EC9706细胞生长及凋亡的影响。方法:采用免疫组织化学SP法检测50例食管鳞状细胞癌组织及15例正常食管组织中mTOR蛋白的表达。分别用不同浓度(20、50和100nmol/L)的rapa处理食管鳞状细胞癌EC9706细胞系,以未处理组作为阴性对照,采用流式细胞术测定细胞周期和凋亡率。结果:食管鳞状细胞癌组织中mTOR蛋白的阳性表达率为64.0%(32/50),高于正常组织的13.3%(2/15)(χ2=11.873,P=0.001)。与对照组比较,rapa可使细胞停滞于G0/G1期(F=102.609,P=0.001),且明显促进细胞凋亡(F=916.882,P=0.001)。结论:mTOR信号通路在食管鳞状细胞癌中被显著激活,rapa能抑制食管鳞状细胞癌细胞生长并诱导细胞凋亡。
Aim：To study the activation of mammalian target of rapamycin（mTOR） signaling pathway in esophageal squamous cell carcinoma （ESCC） and the effects of rapamycin on the cell growth and apoptosis of ESCC cell line EC9706.Methods：The expressions of mTOR in 50 ESCC tissues and 15 normal esophageal tissues were detected by immunohistochemistry. The cell cycle and cell apoptosis were determined by using flow cytometry in EC9706 cells treated with rapamycin at different concentrations （20,50 and 100 nmol/L）,and the untreated cells were used as control.Results：The expression rate of mTOR in ESCC tissues was 64.0%（32/50）,higher than that in normal esophageal tissues,and there was obvious difference（χ2=11.873,P=0.001）.Compared with control groups,the EC9706 cells were restrained in G0/G1 phase by rapamycin （F=102.609,P=0.001）;rapamycin induced apoptosis of the EC9706 cells （F=916.88.609,P=0.001）.Conclusion：mTOR signaling pathway is activated in the ESCC and rapamycin can inhibit the growth and induce apoptosis of ESCC cells.
出处
《郑州大学学报（医学版）》
CAS
北大核心
2010年第3期356-359,共4页
Journal of Zhengzhou University(Medical Sciences)
基金
国家自然科学基金资助项目30901778
教育部"十.五""211工程"重点学科建设项目教重字2002-2
