摘要
目的:探讨丙戊酸钠(VPA)对体外培养的人喉癌Hep-2细胞凋亡的影响及其机制。方法:不同浓度VPA处理人喉癌Hep-2细胞不同时间后,采用MTT法检测细胞的增殖活性,流式细胞仪检测凋亡率,RT-PCR检测凋亡抑制蛋白Survivin mRNA表达的变化。结果:VPA对人喉癌Hep-2细胞的生长具有明显的增殖抑制作用,其作用表现为剂量依赖性和时间依赖性(P<0.01)。流式细胞仪检测发现:以3 mmol/L的VPA处理Hep-2细胞后,其凋亡率呈时间依赖性上升(P<0.01)。RT-PCR检测发现人喉癌Hep-2细胞Survivin mRNA表达呈时间依赖性下调(P<0.01)。结论:VPA对人喉癌Hep-2细胞具有明显的增殖抑制和诱导凋亡的作用,其作用机制与下调Survivin表达比例有关。
Objective：To observe the effect of sodium valproate（VPA） on proliferation and apoptosis of human laryngeal carcinoma Hep-2 cells and its associated mechanism.Method：Methabenzthiazuron（MTT） was used to observe the proliferation of human laryngeal carcinoma Hep-2 cells treated with various concentrations of VPA at different times.Flow cytometry（FCM） and RT-PCR were used to measure the apoptosis rate and the expression of Survivin mRNA in the Hep-2 cells treated with VPA at 3mmol/L for different times.Result：VPA inhibited growth of Hep-2 cells in a dose-dependent and time-dependent manner（P〈0.01）.The apoptosis rate increased after the treatment by VPA at 3 mmol/L.There were significant differences between different time groups（P〈0.01）.The expression of Survivin mRNA of Hep-2 cells were decreased in a time dependent manner（3 mmol/L）（P〈0.01）.Conclusion：VPA have obvious growth inhibition and induction of apoptosis on human laryngeal cancer Hep-2 cells,its mechanism is related to decrease the expression of Survivin.
出处
《临床耳鼻咽喉头颈外科杂志》
CAS
CSCD
北大核心
2010年第8期359-362,共4页
Journal of Clinical Otorhinolaryngology Head And Neck Surgery
基金
河南省医学高新技术发展扶持重点项目(No:200802005)
关键词
喉肿瘤
丙戊酸钠
增殖
凋亡
SURVIVIN
laryngeal neoplasms
sodium valproate
roliferation
apoptosis
Survivin
