摘要
脂肪酸的含量是油料作物重要的加工指标之一,而目前的脂肪酸测定方法步骤繁琐、耗时长、需要样品量大,育种工作者要对大批的后代材料进行检测是非常困难的。并且因为籽粒粉碎后不能延续后代,在待检测材料种子量很少时,常遇到检测与繁种的矛盾。笔者建立了一种微量、快捷的花生脂肪酸检测方法,使花生脂肪酸检测变的非常简便。从花生籽粒上切取0.020g,采用提取液(苯:石油醚=1:1)提取5min,0.5mol/L甲醇钠酯化10min后即可进行气相色谱分析,使以往需要用几个小时的工作在15min内完成,大大缩短了检测时间。通过与传统的索氏提取(GB/T5009.6—2003)、甲酯化方法(GB/T17376—1998)比较,该方法结果准确可靠。
The content of fatty acids is an important index for oil crops. The traditional methods of detecting fatty acids need more samples and more time, which is difficult to select hundreds of materials for breeders. To detect the fatty acids or to propagate is ambivalent when the material detected has few seeds. In this experiment, a fast microanalysis was studied for detecting fatty acids of peanut. A part of about 0.020 g cut from one seed of peanut was used, extracted oil using the mixture （benzene： petroleum ether=-1：1） for 5 minutes, and methyl esterificated using sodium hydroxide-methanol solution （c-：- 0.5 tool/L） for 10 minutes, then the supernatant fluid was injected into the GC system. Using this method, the work which usually need several hours, now can be completed in 15 min, thus lots of time can be saved. By comparing this method with traditional method （GB/T 5009.6-2003, GB/T 17376-1998） for detecting fatty acid compositions, it was confirmed that the results got from this method was exact and credible.
出处
《中国农学通报》
CSCD
北大核心
2010年第13期98-103,共6页
Chinese Agricultural Science Bulletin
关键词
花生
脂肪酸
微量检测
气相色谱
soybean, fatty acids, microanalysis, gas chromatography
