摘要
针对乳制品、糕点、酒类、饮料、糖果等食品中添加的功能活性成分低聚果糖,包括蔗果三糖（GF2）、蔗果四糖（GF3）、蔗果五糖（GF4）的检测,建立了水和乙腈为流动相,梯度洗脱,氨基色谱柱分离,蒸发光散射检测器（evaporativelight-scattering detector,ELSD）检测食品中添加的低聚果糖含量的高效液相色谱方法。蔗果三糖、蔗果四糖、蔗果五糖的线性工作范围分别为0·04~0·45、0·05~0·50、0·04~0·45mg/mL,线性相关系数R2分别为0·9999、0·9999、1。对不同类型的食品,三种低聚果糖的回收率在90·0%~110·0%之间,方法准确度高。室内重复性变异系数一般都低于10%,室间再现性变异系数一般都低于15%,说明方法的重复性和再现性良好。该方法简单、易于掌握,测定结果准确,适于复杂食品基质中低聚果糖含量的测定。
For the determination of fructooligosaccharides in foods such as dairy products,cake,drinking and candy,a high performance liquid chromatography method was established.GF2（1-ketose）,GF3（nystose） and GF4（1F-fructofruranosyl nystose） in various foods were separated on a NH2 column by gradient elution and detected by evaporative light-scattering detector（ELSD）.The linear ranges of the calibration curves for GF2,GF3 and GF4 were 0.04 ~ 0.45,0.05 ~ 0.50 and 0.04 ~ 0.45mg/mL,respectively.The linear correlation coefficients（R2） were 0.9999,0.9999 and 1,respectively.As to different kinds of foods,the spike recoveries were in the range of 90.0% ~110.0%.Coefficient variability of repeatability and reproducibility were lower than 10% and 15%,respectively.The method was simple and was apt to be grasped,can be used in the quantification of fructooligosaccharides in complex food matrix.
出处
《食品工业科技》
CAS
CSCD
北大核心
2010年第9期370-374,共5页
Science and Technology of Food Industry
基金
国家标准化管理委员会国标委(20071140-T-469)
关键词
食品
低聚果糖
高效液相色谱法
含量测定
food
fructooligosaccharides
high performance liquid chromatography
content determination
