摘要
以秦皇岛海域的缢蛏为原料,通过不同提取方法的比较,确定超声辅助酶解提取法为较优的多糖提取方法,并通过响应面法对缢蛏多糖提取工艺进行优化。试验结果表明,原料超声预处理30min后再进行酶解提取,在添加3.99%的木瓜蛋白酶、50.9℃、酶提8.94h条件下缢蛏多糖提取量达9.41mg/g(鲜)。该方法对研究海水贝类多糖功能性产品原料的选取及产品的开发与应用有重要意义。
Razor clam polysaccharide with various biological activity was rich in polysaccharide functional product.Establishment of more economical and reasonable experimental design through maths model,it was very important signification for exploitation and application of razor clam polysaccharide.The research carried out in the Qinhangdao sea showed that the supersonic assistance enzymolysis extraction was found to be the preferable polysaccharide extraction method for the razor clam polysaccharide extraction by the response surface method.The results indicated that the feasible extraction condition was as the following：30 min of supersonic assistance extract time was,amount of papain of 3.99%,extract Temperature of 50.9 ℃,extract time of 8.94 h.Under this condition the razor clam polysaccharide extraction content was achieved at a rate of the 9.41 mg/g fresh weight.This research has the vital significance regarding the marine shellfish polysaccharide function product raw material selection and the product development and the application.
出处
《水产科学》
CAS
北大核心
2010年第4期203-207,共5页
Fisheries Science
基金
国家海洋公益性行业科研专项(200805046)
关键词
缢蛏
多糖提取
响应面分析法
razor clam
polysaccharide extraction
response surface method analysis
