摘要
研究了N/P(1∶1、5∶1、12.5∶1、30∶1、50∶1、80∶1)对菱形藻的生长速率、营养成分含量及氮磷利用率的影响。试验结果表明,(1)N/P对菱形藻的生长影响显著(P<0.05),其中N/P为12.5∶1时,比生长速率最大,当N/P<5∶1和N/P>50∶1时,菱形藻的生长较慢,比生长速率为0.3～0.6/d;(2)藻细胞营养成分的含量也受N/P的影响:在N/P为12.5∶1时,叶绿素的含量达到1.81g/ml,总脂肪、蛋白质、胞外多糖和胞内多糖的含量分别占干质量的29.9%、17.8%、15.3%、14.2%,高于其他N/P下的营养成分含量;(3)培养后水体中氮磷的含量明显下降,TN的利用率之间有显著性差异(P<0.05),利用率均超过55%,最高为30∶1,达78%,TP的利用率之间无显著性差异(P>0.05),均高于90%。
The growth and nutrient contents in alga Nitzschia sp.were studied under different ratios of nitrogen to phosphorus（1∶1,5∶1,12.5∶1,30∶1,50∶1,and 80∶1）to evaluate the uptake ratio of nitrogen to phosphorus.It was found that there was significant differences in growth rate（P0.05）,the maximum at N/P=12.5∶1,and the minimum at N/P5∶1 and N/P50∶1.The nutrient contents were obviously in accordance with the growth.There were chlorophyll c（P0.05）ontent of 1.81 g/ml,29.9% of extracellular polymeric substance（EPS）,17.8% of intracellular polymeric substance（IPS）,5.3% of protein and 14.2% of lipid in the alga at N/P=12.5∶1,higher than those at other ratios of nitrogen to phosphorus.After being cultivated,there was much lower N and P levels in the water media after culture,indicating that there were significant differences in the uptake ratio of total nitrogen（TN）,over 55%（the maximum of 78% at N/P=30∶1）（P0.05）.However,there were no significant differences in the uptake ratio of total phosphorus（TP）,over 90%.
出处
《水产科学》
CAS
北大核心
2010年第4期198-202,共5页
Fisheries Science
基金
山东省黄河三角洲生态重点实验室开放基金资助项目(2009KFJJ02)
南京农业大学江苏省海洋生物学重点实验室研究基金资助项目(JSMK2008-004)
中国科学院海洋生物资源可持续利用重点实验室(LMB)
广东省应用海洋生物学重点实验室(LAMB)
广东省海洋生物重点实验室(LMM)开放课题(LMB71005)
关键词
菱形藻
N/P
生长
营养成分
Nitzschia sp.
ratio of nitrogen to phosphorus
growth
nutrient content（P〉0.05）
