摘要
目的:了解P物质及其受体神经激肽1(NK-1)在结肠癌中的表达及定位,探讨其在结肠癌发病及临床诊断的意义。方法:应用免疫组化方法检测正常结肠、结肠癌组织及癌旁组织中SP及NK-1的表达。采用jmtjfx10[1].31统计学软件处理数据,所得数据进行Q检验。结果:①在结肠癌组织中P物质阳性着色于胞浆,呈巢状或弥漫分布。②NK-1受体主要着色于细胞浆内,呈巢状或弥漫分布;少数为于细胞膜。③p物质与其受体NK-1在正常结肠粘膜组织及癌旁组织也有表达,位于细胞浆内,形态多呈圆形、椭圆形或不规则形。④二者在绝大多数结肠组织中呈强阳性表达,显著高于正常结肠粘膜组织和癌旁组织(p<0.01).结论:p物质与其受体NK-1在结肠癌组织中高度表达,提示神经内分泌参与了结肠癌的发生有关,说明P物质及NK-1受体可能参与了结肠癌的发病过程。
Objective：To investigate the expression of substance P and receptor neurokinin-1 in human colon cancer and its clinical significance.Methods：Immunohistochemistry was used to detect the expression of substance P and neurokinin-1 in normal specimens,adjacent specimens and adenocarcinoma specimens in colon.Using jmtjfx10 [1].31 statistical software to process data and the data.obtained for Q test..Result：①Substance P,showed a diffuse or nest-like cytoplasmic staining,was mostly localized within the cytoplasm by Immunohistochemistry。 ② The high distribution of receptor neurokinin-1 expression,showed a diffuse or nest-like cytoplasmic staining but a few in membrane,was observed in colon cancer by Immunohistochemistry。③Substance p and receptor neurokinin-1 were also distributed in the cytoplasm in normal colorectal mucosal specimens and adjacent specimens.④The expression of substance P and receptor neurokinin-1 in colon cancer was significantly higher than that in normal specimens and adjacent specimens（p0.01）.Conclusion：The high expression of substance p and receptor neurokinin-1 in colon adenocarcinoma tissues suggests the neuroendocrine mechanism involves in colon cancer development.
出处
《现代生物医学进展》
CAS
2010年第8期1515-1517,共3页
Progress in Modern Biomedicine
基金
Qingdao Municipal Science and Technology Development Fund Projec(t08-2-1-4-nsh)~~
关键词
结肠癌
P物质
受体
神经激肽1
免疫组织化学
Colon cancer
Substance P（SP）
neurokinin-1（NK-1 Receptor）
Immunohistochemistry
