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结肠癌RKO细胞P物质和NK-1免疫细胞化学检测

IMMUNOCYTOCHEMICAL EXPRESSIONS OF SUBSTANCE P AND NEUROKININ-1 IN RKO CELLS OF COLON CANCER
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摘要 目的探讨P物质及其受体神经激肽1(NK-1)在体外培养的结肠癌细胞系RKO中的表达及免疫细胞化学定位。方法将RKO细胞接种于提前放入盖玻片的6孔板进行细胞培养,细胞融合30%时将盖玻片取出进行免疫细胞化学染色。结果在RKO细胞系中P物质和NK-1呈阳性表达,P物质阳性表达多数位于细胞浆;而NK-1阳性表达多数位于细胞浆,少数位于细胞膜。结论神经内分泌机制可能参与了结肠癌的发病过程。 Objective To investigate the immuno-expressions of substance P and NK-1 in RKO cell lines of colon cancer.Methods RKO cells were inoculated on the cover glass and cultured on 6-well plate.The cover glass was removed from the plate upon 30% of cell fusion,and the expressions of SP and NK-1 of the cultured cells were checked via immunocytochemical staining.Results Positive expressions of substance P and NK-1 were mainly found within the cytoplasm of the RKO cells,while a few of NK-1 noted on the membrane. Conclusion The neuroendocrine mechanism might involve in the process of morbidity of colon cancer.
作者 赵岭岭 陈桦 王继刚
机构地区 青岛大学医学院附属青岛市市立医院 青岛大学医学院附属医院病理科
出处 《齐鲁医学杂志》 2010年第4期289-290,293,共3页 Medical Journal of Qilu
基金 青岛市科技发展计划基金资助项目(08-2-1-4-nsh)
关键词 结肠肿瘤 RKO细胞系 P物质 受体 神经激肽 免疫细胞化学 colonic neoplasms RKO cell line substance P receptors neurokinin-1 immunocytochemistry
分类号 R737.9 [医药卫生—肿瘤]
  • 相关文献

参考文献14

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二级参考文献25

  • 1LEEMAN S E,FERGUSON S L.Substance P:an historical perspective[J].Neuropeptides,2000,34(5):249-254.
  • 2BEAUJOUAN J C,TORRENS Y,SAFFROY M,et al.A 25 year adventure in the field of tachykinins[J].Peptides,2004,25(3):339-357.
  • 3LUO W,SHARIF T R,SHARIF M.Substance P-induced mitogenesis in human astrocytoma cells correlates with activation of the mitogen-activated protein kinase signaling pathway[J].Cancer Res,1996,56(21):4983-4991.
  • 4FONG T M,ANDERSON S A,YU H,et al.Differential activation of intracellular effector by two isoforms of human neurokinin-Ⅰ receptor[J].Mol Pharmacol,1992,41(1):24-30.
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  • 7DMTR A,PEIDL Z,VINCZE A,et al.Immunohistochemical distribution of vanilloid receptor,calcitonin-gene related peptide and substance P in gastrointestinal mucosa of patients with different gastrointestinal disorders[J].Inflammopharmacology,2005,13(1-3):161-177.
  • 8RAMESHWAR P,GASCN P.Hematopoietic modulation by the tachykinins[J].Acta Haematol,1997,98(2):59-64.
  • 9NOWICKI M,OSTALSKA-NOWICKA D.The significance of substance P in physiological and malignant haematopoiesis[J].J Clin Pathol,2007,60(7):749-755.
  • 10HORSTMANN S,KAHLE P J,BORASIO G D.Inhibitors of p38 mitogen-activated protein kinase promote neuronal survival in vitro[J].J Neurosci Res,1998,52(4):483-490.

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齐鲁医学杂志
2010年 第4期

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