摘要
采用平板计数法和磷脂脂肪酸(PLFA)分析方法评价了喷施敌敌畏杀虫剂后对桃树叶际微生物群落的影响。平板计数法分析结果表明,经80%敌敌畏乳油的1000倍液喷雾处理后,可培养微生物数量低于喷水对照。PLFA分析结果显示,桃树叶际真菌标记物18:1ω9t磷脂脂肪酸(PLFAs)含量最高,超过总PLFAs含量的60%;喷施杀虫剂后,叶际微生物PLFAs的含量、种类均有所增加,明显有别于喷水对照;并且增加敌敌畏处理次数会增强其对叶际微生物群落影响的显著性。PLFAs主成分分析表明,处理1d后不同样品的叶际微生物群落结构差异最明显,7d后,不同处理样品的叶际微生物群落结构差异变小。
Although dichlorovos（DDVP）is one of the most widely used organophosphorus insecticides,little is known regarding the impacts of applying the insecticide on the microbial community in plant phyllosphere.In the present study,the impact of DDVP treatment on the microbial community of peach phyllosphere was assessed using the culture-dependent menthod of dilution-plate counting and the culture-independent technique of phospholipid fatty acid（PLFA）analysis.Dilution-plate counting assay indicated that the number of culturable microorganisms in peach phyllosphere treated by DDVP insecticide was less than that in the water treatment.Whereas,PLFA analysis suggested that DDVP insecticide treatment led to a significant increased in both total biomass and species.PLFA profiles also indicated that the fungal unsaturated PLFA（18：1ω9t）was predominant（60% of the total PLFAs）in peach phyllosphere.Principal component analysis of PLFAs data indicated that DDVP treatment did change the phyllosphere microbial community structure significantly;the phyllosphere microbial communities of DDVP and water treatments were the most different after 1 day treatment,and became similar after 7 days.
出处
《农业环境科学学报》
CAS
CSCD
北大核心
2010年第5期864-869,共6页
Journal of Agro-Environment Science
基金
国家自然科学基金项目(30600082)
国家科技支撑计划项目(2007BAC27B01)
中国科学院知识创新工程重大项目(kzcx1-yw-06-03)
关键词
桃树
叶际
敌敌畏
微生物群落
磷脂脂肪酸
Prunus persica
phyllosphere
dichlorovos
microbial community
phospholipid fatty acid
