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分子生态技术在污染土壤微生物生态研究中的应用

Application of Molecular Ecological Techniques in Research of Microbial Diversity in Contaminative Soil
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摘要 分子生态学技术克服了传统微生物培养技术的限制,可以更精确地揭示环境中微生物种群结构及其遗传的多样性,使得污染微生物生态学的研究进入一个新的阶段.本文主要介绍了基于16S rDNA的分子分析技术,包括16S rD-NA序列分析、ARDRA((扩增rDNA限制性分析)、T-RFLP、RFLP(末端限制性片段长度多态性)技术、RISA(核糖体基因间隔序列分析)法、SSCP(单链构象多态性)分析、DGGE/TGGE(变性/温度梯度凝胶电泳)技术、RAPD(随机扩增多态性DNA分子标记)技术,及其在污染环境微生物多样性研究中的应用研究进展,为污染微生物生态学的进一步研究应用提供帮助. Molecular ecological techniques resolve lots of difficulties in studying the microorganisms which could not be cultured. The research of pollution microbi - al ecology gains a new phase. This paper presents a mini-review on the molecular ecological techniques used for mierobial diversity research in contaminative environment based on 16S rDNA, as well as 16S rDNA sequence analysis, ARDRA （Amplitied rDNA Restriction Analysis）, T-RFLP/RFLP （terminal restriction fragment length polymorphism）, RISA （ ribosomal intergenic spacer analysis）, SSCP（single strand conformational polymorphism）,DGGE/TGGE （Denaturing/Temperature gradient gel eletrophoresis） and RAPD （Random Amplified Polymorphism DNA）.
作者 段学军
机构地区 中原工学院
出处 《中原工学院学报》 CAS 2006年第1期26-30,40,共6页 Journal of Zhongyuan University of Technology
基金 河南省教育厅自然科学基金(200510465005)
关键词 分子生态技术 污染环境 微生物多样性 molecule ecology technique contaminative environment biodiversity
分类号 Q938 [生物学—微生物学] X171 [环境科学与工程—环境科学]
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参考文献30

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中原工学院学报
2006年 第1期

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