摘要
目的建立百合知母汤水煎液中总黄酮和总皂苷含量测定方法。方法 :采用紫外分光光度法对知母总黄酮和总皂苷进行测定。结果 :芒果苷线性关系为y=0.0702x-0.1189,加样回收率为98.55%,RSD=1.65%(n=5);菝葜皂苷元线性关系为y=0.0236x+0.017,加样回收率为97.11%,RSD=2.82%(n=5)。结论 :该方法简便、快速、准确,适合百合知母汤中总黄酮和总皂苷含量的测定,可以作为该方的质量控制方法 。
OBJECTIVE To estable a method of determining total flavonoids and total saponins for Baihezhimu Decoction. METHODS Determination of tatol flavonoids and total saponins were used by UV -- spectrophotometry. RESULTS mangiferin is y = 0.0702x -0.1189, Recoverage is 98.55%, RSD = 1.65% （n=5） . The linear of sarsasapogenin is y = 0. 0236x ＋ 0.017, Reeoverage is 97.11 %, RSD= 2.820% （n = 5） . CONCLUSIONS The method is simple, quick, and accurated, and suitable to determine content of total flavonoids and saponins for Baihezhimu Decoction.
出处
《南京中医药大学学报》
CAS
CSCD
北大核心
2010年第3期223-225,共3页
Journal of Nanjing University of Traditional Chinese Medicine
基金
国家自然科学基金重大研究计划项目(90709025)
关键词
知母
百合
总黄酮
总皂苷
含量测定
Lilium laneifolium Thunb.
Anemarrhena asphodeloides Bge
total flavonoids
total saponins
determination
