摘要
以罗红霉素为电荷给体,亚甲基蓝为电子受体,分光光度法研究了它们之间形成电荷转移络合物的条件。结果表明:在乙醇-盐酸介质中,罗红霉素与亚甲基蓝于室温条件下形成稳定的络合物,其最大吸收波长为666nm,罗红霉素在30.14—66.30mg/L范围服从Beer定律,检出限为9.33mg/L,表观摩尔系数ε=2.01×103L·mol-1.cm-1。罗红霉素与亚甲基蓝的络合比为2∶1,并对形成络合物的机理进行了初步的探讨。方法用于罗红霉素片剂和胶囊样品中罗红霉素含量的检测,结果满意。
Spectrophotometry for the determination of roxithromycin has been developed based on the charge transfer reaction.The reaction between roxithromycin and methylene blue was completed in alcohol-HCl medium at room temperature.The maximum absorption wavelength was at 666nm.Beer＇s law was obeyed in the range of 30.14—66.30mg/L of roxithromycin,and the apparent molar absorption coefficient was 2.01×103L·mol-1·cm-1.The detection limit was 9.33mg/L.The composition of the complex was found to be 2：1.The mechanism of reaction was investigated.The method has been applied to the determination of roxithromycin in tablet and capsule with satisfactory results.
出处
《光谱实验室》
CAS
CSCD
北大核心
2010年第3期1085-1088,共4页
Chinese Journal of Spectroscopy Laboratory
基金
广西教育厅科研项目(200807LX381)经费资助
关键词
荷移反应
亚甲基蓝
罗红霉素
分光光度法
Charge Transfer Reaction
Methylene Blue
Roxithromycin
Spectrophotometry
