摘要
采用霍恩氏法进行对虾保鲜剂对小白鼠的急性毒性试验研究,结果表明:对虾保鲜剂对雌雄小鼠半数致死量(LD50值)>10g/kg体重,毒性分级属实际无毒级。在加与不加代谢活化系统(S-9)的条件下,利用4个组氨酸营养缺陷型鼠伤寒沙门氏菌菌株(TA97、TA98、TA100、TA102),采用平板渗入进行鼠伤寒沙门氏菌哺乳动物微粒体酶试验(Salmonella typhimurium mammals microsomal enzyme test,Ames),研究对虾保鲜剂的致突变作用,结果表明:对虾保鲜剂对4个菌株诱发的回变菌落数与阴性对照组的回变菌落数之间没有显著差异(P>0.05),均未超过阴性对照组2倍,Ames试验结果为阴性,表明对虾保鲜剂对鼠伤寒沙门氏菌无直接和间接致突变作用。
The acute toxicity test of prawn preserving reagent to mice was studied by Horn＇s.The test revealed that median lethal dose（LD50）of prawn preserving reagent in mice was 10 g/kg.According to the acute toxicity standard rules,the test object was practically non-toxic grade.The Ames test of prawn preserving reagent to Salmonella typhimurium histamine-auxotroph mutants（TA97、TA98、TA100、TA102）was conducted.The revertant colonies of test groups were not two times more than negative control groups＇.There were not significant differences（P0.05）between groups.The result of the Ames test was negative and showed the sample of Prawn preserving reagent did not contain any direct or indirect-acting mutagenicity to the test strains.
出处
《上海海洋大学学报》
CAS
CSCD
北大核心
2010年第3期410-414,共5页
Journal of Shanghai Ocean University
基金
浙江省科技计划项目(2007F70048)
