摘要
目的建立胶囊和血样中洛美沙星的含量测定方法。方法在醋酸-醋酸钠缓冲液(pH=6.0)中,铽离子(Tb3+)能与洛美沙星反应形成络合物,该络合物在紫外光照射下能发射铽的特征荧光,λex=320nm,λem=545nm,狭缝均为5nm。结果浓度线性范围为1.0×10-8～2.4×10-6mol/L,回归方程为F=6.4487×108C-5.9856,r=0.9999(n=7),检出限为3.5ng/mL。结论所用方法简单、快速、灵敏,可用于血样及洛美沙星胶囊的含量测定。
Objective To establish a method to measure the lomefloxacin（LFLX）in capsule and blood.Methods In the HAc-NaAc buffer solution（pH=6.0）,Tb^3＋-LFLX complex could be formed.The fluorescence emission of Tb3＋could be detected after the absorption of ultraviolet light by the complex.λex=320 nm,λem=545 nm,and the slit widths were both 5 nm.Results Under the optimal experimental conditions,the linear range of the determination was 1.0×10^-8-2.4×10^-6 mol/L,the regression equation was F=6.448 7×10^8C-5.985 6（r=0.999 9）,and the detection limit was 3.5 ng/mL for LFLX.Conclusion The method with simplicity,convenience,rapidness and sensitivity can be successfully applied to the determination of lomefloxacin in pharmaceuticals and biological fluids.
出处
《中国药业》
CAS
2010年第9期23-25,共3页
China Pharmaceuticals
关键词
铽
洛美沙星
荧光增敏
含量测定
terbium
lomefloxacin
fluorescence enhancement
content determination
