摘要
目的：建立洛美沙星人血浆及尿药浓度ＨＰＬＣ柱切换测定法。方法：ＬｉＣｈｒｏｐｒｅｄＲＰ２（３０ｍｍ×４ｍｍ，２５～４０μｍ）预处理柱，水为预处理流动相。ＳｈｉｍｐａｃｋＣＬＣ－ＯＤＳ分析柱（１５０ｍｍ×６ｍｍ，５μｍ），分析流动相为甲醇－０．２ｍｏｌ／Ｌ醋酸铵（ｐＨ２．０）（３２６８测血浆，３４６６测尿），紫外检测波长２９０ｎｍ。结果：洛美沙星血浆及尿最低检测浓度分别为０．１ｍｇ／Ｌ及５ｍｇ／Ｌ，线性范围０．１２５～６ｍｇ／Ｌ及５０～１０００ｍｇ／Ｌ，平均净化回收率７４．２％±２．２９％及８９３％±１．５２％。结论：用于测定８名健康志愿者口服洛美沙星制剂４００ｍｇ后血浆及尿药浓度。
OBJECTIVE:To establish a simple HPLC column switching method for measuring lomefloxacin in human plasma and urine.METHOD:Diluted plasma and urine were directly injected into the pretreatment column of LiChropred RP 2(30 mm×4 mm,25～40 μm),and were cleaned up by water.The Shimpack CLC ODS(150 mm×6 mm,5 μm) was used as analytical column.The mobile phase consisted of methanol 0.2 mol/L ammomiun acetate buffer(pH 2.0)(32∶68 for plasma,34∶66 for urine).The eluent was monitored at 290 nm.RESULTS:The limit of detection was 0.1 mg/L and 5 mg/L,linear range was 0.125～6 mg/L and 50～1 000 mg/L,the recovery of clean up was 74.2%±2.29% and 89.3%±1.52%,for lomefloxacin in human plasma and urine respectivly.CONCLUSION:This method was applied to the determination of lomefloxacin in eight Chinese healthy volunteers after a single oral dose of 400 mg.
出处
《中国药学杂志》
CAS
CSCD
北大核心
1996年第7期417-420,共4页
Chinese Pharmaceutical Journal
