摘要
【目的】利用一株经体外筛选具有较强抗氧化活性的干酪乳杆菌干酪亚种(Lactobacillus casei subsp.casei)SY13活菌制剂和灭活制剂研究D-半乳糖所致衰老模型小鼠体内抗氧化能力。【方法】采用SY13活菌制剂和灭活制剂饲喂由D-半乳糖连续皮下注射诱导的亚急性衰老模型小鼠,以各组小鼠血清和肝组织匀浆中超氧化物岐化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)的活力、总抗氧化能力(T-AOC)及脂质过氧化产物丙二醛(MDA)的含量为指标,全面评价其在小鼠体内的抗氧化能力。【结果】Lactobacillus casei subsp.casei SY13活菌制剂高、中剂量组(20、10mL·kg-1)能降低连续6周注射D-半乳糖诱发的衰老小鼠血清和肝脏组织中的MDA含量,同时提高血清和肝脏组织中GSH-Px活力以及总抗氧化能力(T-AOC),SOD活力变化不明显。【结论】SY13活菌制剂能有效提高亚急性衰老小鼠的抗氧化能力,具有一定延缓衰老的作用。
【Objective】 The objective of the experiment was to study the antioxidative activity of viable cells and heat-killing cells of Lactobacillus casei subsp.casei SY13 on aged model mice induced by D-galactose. 【Method】 ICR subacute aged model mice,which were given subcutaneous injection of D-galactose continuously,were fed with viable cells and heat-killing cells of Lactobacillus casei subsp.casei SY13. The content of malondialdehyde （MDA）,activity of superoxide dismutase （SOD） ,glutathione peroxidase （GSH-Px） and total antioxidant capacity （T-AOC） in serum and liver of the mice were tested. 【Result】 SY13 viable cells high dose group and middle dose group（20,10 mL·kg^-1） could obviously reduce the aged mice serum and liver tissue MDA content ,enhance evidently the GSH-Px activity and total antioxidant capacity （T-AOC）,but the SOD activity did not change significantly. 【Conclusion】Lactobacillus casei subsp. casei SY13 viable cells can obviously increase the antioxidant ability of ageing mice and has certain anti-aging effects.
出处
《中国农业科学》
CAS
CSCD
北大核心
2010年第10期2141-2146,共6页
Scientia Agricultura Sinica
基金
国家“863”计划项目(2006AA10Z345)
关键词
干酪乳杆菌
D-半乳糖
衰老小鼠
体内抗氧化
Lactobacillus casei
D-galactose
aged mice
antioxidative activity in vivo
