摘要
利用遗传学方法对分离自内蒙古锡林郭勒盟牧区采集的酸马奶样品中的乳杆菌Lb.casei.Zhang和ZL12-1进行16SrDNA基因扩增与测序,并将结果与同属的乳杆菌作同源性分析,建立系统发育树,以求更准确地确定其归属,为益生菌的筛选奠定基础。结果表明,菌株Lb.casei.Zhang与标准菌株Lb.caseiATCC334T的同源性为100%,菌株ZL12-1与标准菌株Lb.gallinarumATCC33199T的同源性为98%。结合系统发育树及16SrDNA的部分序列分析结果,将Lb.casei.Zhang判定为Lb.caseisubsp.casei,ZL12-1鉴定为Lb.gallinarum,这与传统分类方法所得鉴定结果一致。
To further differentiate Lb.casei.Zhang and ZL12-1 isolated from home-made koumiss in Inner Mongolia, the genes of 16S rDNA from them were amplified in vitro and sequenced. Then a phylogenetic dendrogram was constructed by comparing these two sequences with other 16S rDNA sequences of Lactobacillus. Results indicated that the homology of Lb.casei.Zhang with Lb.casei ATCC 334T was 100%, and the homology of ZL12-1 with Lb.gallinarum ATCC 33199T was 98%. Combined with analysis of phyolgenetic dendrogram and partial sequences of 16S rDNA, Lb.casei.Zhang was classified as Lb.casei subsp.casei , ZL12-1 belonged to Lb.gallinarum. The result was as same as that of traditional classification before.
出处
《中国乳品工业》
CAS
北大核心
2005年第6期4-9,共6页
China Dairy Industry
基金
教育部科学技术研究重点项目(205080)
教育部春晖计划项目(Z2004-2-15009)
