摘要
目的:比较人胃癌细胞系与其腹膜高转移潜能细胞系的基因表达谱,寻找与胃癌腹膜转移相关的基因表达改变。方法:采用基因芯片技术,比较遗传背景相同但转移力有明显差异的两个细胞系GC9811和GC9811-P,分析其基因表达谱的差异。选取部分基因行RT-PCR进一步验证基因芯片的准确性。结果:在11901个候选基因中,筛选出248个(2.1%)差异表达基因。GC9811-P与GC9811相比,表达上调的基因有218个,下调的有30个,包括DNA合成和错配修复基因如H3F3A、细胞增生基因、蛋白合成与修饰基因、信号传导基因和离子通道与运输蛋白相关基因等。半定量RT-PCR对PTEN、S100A4和ZNRD1基因检测结果验证了基因芯片数据的可靠性。结论:胃癌腹膜转移是多基因作用的综合结果,筛选的基因对预测胃癌腹膜转移和抗转移干预措施可能有指导意义。
OBJECTIVE：To study gene expression profile of human gastric carcinoma cell line with peritoneal metastatic potential for screening peritoneal metastasis-related genes.METHODS：The global analysis was performed on differential gene expression of a gastric cancer cell line（GC9811）and its derivative sublines with high potential for metastasis to the peritoneal cavity（GC9811-P）.By applying a high-density oligonucleotide array method,the expressions of approximately 11 901 genes were analyzed,and some selected genes were confirmed by the RT-PCR method.RESULTS：Among the 11 901 target genes,248 genes（2.1%）were differentially expressed,and the up-regulated genes and down-regulated genes were 218 and 30 respectively.The bioinformatical analysis revealed that these genes were closely associated with cell signal transduction,cell metabolize,cytoskeleton,motility,immunity,oncogene and tumor suppressor,cell cycle and cell apoptosis.Real-time PCR showed PTEN,S100A4 and ZNRD1 mRNA expressions in concordance with the results of gene chips.CONCLUSION：cDNA microarray technology is effective in screening differentially expressed genes which may be related to peritoneal metastasis of gastric cancer and supply molecular target gene therapy for clinic.
出处
《中华肿瘤防治杂志》
CAS
2010年第5期321-325,344,共6页
Chinese Journal of Cancer Prevention and Treatment
基金
国家自然科学基金(30160033
30860332)
部分资金来源于教育部春晖计划(Z2006-1-75001)
宁夏回族自治区卫生厅重点项目(2008)
宁夏回族自治区教育厅重点项目(2008)
关键词
细胞系
胃肿瘤
腹膜转移
差异表达基因
基因芯片
cell line
stomach neoplasms
peritoneal metastasis
differential gene expression
cDNA microarray
