摘要
目的:探讨胃癌患者腹膜间皮细胞中ig-h3表达与胃癌生物学行为及腹膜转移相关因素的关系.方法:术中收集75例胃癌和14例胃良性疾病患者下腹前壁腹膜组织,同时收集胃癌患者腹水或腹腔冲洗液,免疫组织化学染色方法检测腹膜间皮细胞中ig-h3含量,RT-PCR方法检测腹水或腹腔冲洗液中CEA mRNA含量,同时进行腹腔冲洗液细胞学(peritoneal lavage cytological,PLC)和病理学检查.结果:29例胃癌和1例胃良性疾病患者腹膜组织ig-h3免疫组织化学染色结果呈阳性,差异具有统计学意义(P=0.030).75例胃癌病例中,13例伴有肉眼腹膜转移,20例腹腔脱落癌细胞呈阳性;32例CEA mRNA阳性.ig-h3阳性率在浸润较深(T4)(P=0.016)、腱状及多彩弥漫的浆膜类型(P=0.037)、肉眼腹膜转移阳性(P=0.002)、腹腔脱落癌细胞阳性(P=0.005)及CEA mRNA阳性组(P=0.027)的患者腹膜组织中表达明显升高,差异具有统计学意义.结论:腹膜间皮细胞中ig-h3表达可作为反映胃癌生物学行为的指标之一,与胃癌腹膜转移密切相关.
AIM： To determine the expression of ig-h3 in peritoneal mesothelial cells in gastric cancer and to explore its relation to pathological parameters and peritoneal metastasis. METHODS： Peritoneal tissue and peritoneal washing fluid samples were collected during operation from 75 patients with gastric cancer and 14 patients with benign gastric lesions. The expression of ig-h3 in peritoneal mesothelial cells was measured immunohistochemically. The mRNA levels of CEA in ascites and peritoneal washing fluid were measured by RT-PCR. Peritoneal lavage cytological （PLC） examination and pathological examination were also performed. RESULTS： ig-h3 was positively expressed in the peritoneal tissue in 29 patients with gastric cancer and 1 with benign gastric lesions, and the positive rate was significantly different between the two groups （P = 0.030）. In the gastric cancer group, 13 patients had evident peritoneal metastasis, 20 were positive for PLC, and 32 were positive for CEA mRNA. The positive rate of ig-h3 expression was significantly positively correlated with deep invasion （P = 0.016）, serious serous type （P = 0.037）, peritoneal metastasis （P = 0.002）, PLC （＋） （P = 0.005）, and CEA mRNA （＋） （P = 0.027）. CONCLUSION： ig-h3 expression in peritoneal mesothelial cells in gastric cancer patients is closely related with peritoneal metastasis, representing a marker of biological behavior of gastric cancer.
出处
《世界华人消化杂志》
CAS
北大核心
2012年第16期1402-1406,共5页
World Chinese Journal of Digestology
基金
国家自然科学基金资助项目
No.81071956~~
关键词
胃癌
腹膜转移
ig-h3
Gastric cancer； Peritoneal metastasis； ig-h3
