摘要
目的 探讨乳腺癌发生过程中细胞核DNA含量变化、cyclinD1、P5 3蛋白表达情况及其相互关系。方法 雌性Wistar大鼠 12 0只 ,利用DMBA 2 0mg·(10 0g) - 1 灌胃 ,复制大鼠乳腺癌动物模型 ,观察正常乳腺、不典型增生乳腺和乳腺癌中细胞核DNA含量变化。应用SP免疫组化技术检测cyclinD1、P5 3蛋白在正常乳腺、不典型增生乳腺及乳腺癌中的表达。结果 在乳腺癌发生过程中 ,正常乳腺、不典型增生乳腺及乳腺癌中细胞核DNA含量增加 (P <0 .0 5 ) ;在不典型增生Ⅰ、Ⅱ、Ⅲ级乳腺组织中分别有 3 2 0、6 2 0、11 2 0有cyclinD1表达 ,7 9的乳腺癌Ⅰ级有cyclinD1的表达 ,1 4的乳腺癌Ⅱ级中cyclinD1有表达。P5 3蛋白在乳癌Ⅰ、Ⅱ、Ⅲ级中分别有 1 9、1 4、6 7有过度表达。结论 在大鼠乳腺癌发生过程中 ,细胞核DNA含量变化反映细胞增殖状态 ,cyclinD1过度表达参与肿瘤的发生过程 ,是肿瘤发生中的早期事件 ,而P5 3表达与肿瘤进展有关 ,是肿瘤发生的晚期事件 ,cyclinD1表达与P5 3蛋白表达呈负相关。
Objective To investigate the interrelation between nuclear DNA content and the expressions of cyclin D1 and P53 in carcinogenesis of breast carcinoma induced by 7,12 dimethylbenz(a)anthracence(DMBA) in rats. Methods Animal model of breast carcinoma was replicated by gastric perfusion of DMBA at 20 mg/100 g. Nuclear DNA content was detected with image analysis instrument and the expressions of cyclin D1 and P53 at every stage of carcinogenesis of breast carcinoma induced by DMBA in rats observed by Sp immunohistochemical staining. Results In mammary gland tissue at grade Ⅰ, grade Ⅱ and grade Ⅲ atypical hyperplasia of breast carcinoma, there was overepression of cyclin D1(3/20, 6/20 and 11/20, respectively). The overexpression of cyclin D1 was detected to be present in grade Ⅰ, grade Ⅱ and grade Ⅲ breast carcinoma (7/9, 1/4 and 0/7, respectively). The overexpression of P53 was detected to be present in grade Ⅰ, grade Ⅱ and grade Ⅲ breast carcinoma (1/9, 1/4 and 6/7, respectively). Conclusion Nuclear DNA content reflects the state of cell proliferation. Cyclin D1 overexpression participates in the carcinogenesis of breast carcinoma, which may be the early event in breast carcinoma. P53 expression is associated with the development of breast carcinoma, which may be the late event in breast carcinoma. The expression of cyclin D1 is negatively correlated with P53 overexpression.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2003年第5期416-419,共4页
Journal of Third Military Medical University
