摘要
目的:研究不同类型的抗肝纤维化药物联合应用即鸡尾酒疗法对大鼠肝星状细胞(HSC)的干扰效果.方法:将体外培养的大鼠肝星状细胞株HSC-T6分为牛磺酸组、表没食子儿茶素没食子酸酯(EGCG)组、4,5,7-三羟基异黄酮组、鸡尾酒组和对照组;各组药物作用HSC-T624h后,用噻唑兰比色法(MTT)测定活细胞数,采用半定量RT-PCR法检测细胞中Ⅰ型胶原及基质金属蛋白酶组织抑制因子-1的mRNA表达水平.结果:MTT实验观察到牛磺酸、EGCG、三羟基异黄酮、鸡尾酒组HSC-T6的活细胞数目减少,与对照组的A值比较有显著性差异(0.237±0.007,0.216±0.009,0.242±0.008,0.130±0.004vs0.452±0.011,均P<0.05),其中鸡尾酒组与单一用药各组相比也有显著性差异(P<0.05).与对照组相比,各组的Col-Ⅰ、TIMP-1的mRNA表达降低,并且鸡尾酒组与单一用药各组相比有统计学意义(P<0.05).结论:鸡尾酒疗法可抑制大鼠HSC-T6的增殖,与单一用药相比更能有效降低Col-Ⅰ、TIMP-1的mRNA表达,可能具有更高的纤维化效果.
AIM： To investigate the effects of cocktail therapy on cell proliferation and collagen I （Col I） and tissue inhibitor of metalloproteinase-1 （TIMP-1） mRNA expression in rat hepatic stellate cells. METHODS： Hepatic stellate cells （HSC-T6） cultured in vitro were exposed to taurine （taurine group）, epigallocatechin gallate （EGCG; EGCG group）, genistein （genistein group）, and a combination of these three drugs （cocktail-treated group） for 24 h, respectively. Cell proliferation was determined by methyl thiazol tetrazolium （MTT） assay. The expression of Col I and TIMP-1 mRNAs was measured by semi-quantitative reverse-transcription polymerase chain reaction （RT-PCR）. RESULTS： Cell proliferation was significantly inhibited in the taurine group, EGCG group, genistein group and cocktail-treated group compared with the control group （0.237 ± 0.007, 0.216 ± 0.009, 0.242 ± 0.008 and 0.130 ± 0.004 vs 0.452 ± 0.011, respectively; all P 0.05）. The reduced rate of cell proliferation was more significant in the cocktail-treated group than in other treatment groups （all P 0.05）. The expression levels of Col I and TIMP-1 mRNAs markedly decreased in all treatment groups when compared with the control group （all P 0.05）. The expres sion levels of Col I and TIMP-1 mRNAs were significantly lower in the cocktail-treated group than in other treatment groups （P 0.05）. CONCLUSION： Cocktail therapy significantly inhibits cell proliferation and down-regulates Col I and TIMP-1 mRNA expression in HSCs. Cocktail therapy may be more effective than monotherapy in the treatment of liver fibrosis.
出处
《世界华人消化杂志》
CAS
北大核心
2010年第9期932-936,共5页
World Chinese Journal of Digestology
基金
广西地方性高发疾病防治研究重点实验室开放基金资助项目
No.0842009-K5~~
关键词
鸡尾酒疗法
肝星状细胞
基因表达
Cocktail therapy
Hepatic stellate cell
Gene expression
