摘要
目的应用核磁共振氢谱(1Hnuclear magnetic resonance,1HNMR)和主成分分析(principal componentanalysis,PCA)方法研究乙肝后肝硬化患者血清的代谢组变化。方法慢性乙肝患者血清20例和乙肝后肝硬化患者血清18例,采用BRUKER Avance 500 MHz超导核磁共振波谱仪进行检测,并运用模式识别方法分析乙肝后肝硬化患者血清代谢组变化。结果乙肝患者和乙肝后肝硬化患者血清的代谢组具有明显的差异。肝硬化引起血清中的脂和胆碱/磷酸胆碱降低,而乳酸、丙氨酸、甲硫氨酸、谷氨酰胺、肌酸以及葡萄糖的含量升高。结论通过上述代谢物的变化可以区分乙肝和乙肝后肝硬化患者。这种基于磁共振氢谱和主成分分析的代谢组学方法可以为肝硬化的诊断提供可靠的分子水平上的代谢依据。
Objective 1H Nuclear magnetic resonance（1H NMR） spectroscopy in conjunction with principal component analysis（PCA） had been employed to characterize the metabolite composition of the serum samples from the patients with posthepatitic cirrhosis.Methods 1H NMR spectra were measured for serum samples from 20 patients with chronic hepatitis B and 18 patients with posthepatitic cirrhosis,and the spectra data were analyzed by PCA to reveal the metabonomic changes.Results There were clear metabonomic differences between the patients with chronic hepatitis B and the patients with posthepatitic cirrhosis that could lead to decreased levels in lipids and phosphocholine/choline,and escalating levels of lactate,alanine,methionine,glutamine,creatine and glucose.Conclusion The results showed that the metabolite changes of serum from the patients with posthepatitic cirrhosis were markedly different from those of the patients with chronic hepatitis B,what indicated that 1H NMR-and principal component analysis-based metabonomic technique could provide an efficient tool for the diagnosis of posthepatitic cirrhosis at molecular level.
出处
《广东药学院学报》
CAS
2010年第1期99-101,共3页
Academic Journal of Guangdong College of Pharmacy
基金
广东药学院人才建设专项基金
博士启动基金(43543109)
关键词
磁共振氢谱
肝硬化
代谢
主成分分析
proton nuclear magnetic resonance
posthepatitic cirrhosis
metabonomics
principal component analysis
