摘要
为了调查禾谷类孢囊线虫（cereal cyst nematode,CCN）等在安徽省小麦根际发生种类和分布,利用＂zig-zag＂法对萧县等有代表性的24个县市的小麦地进行土壤平面或分层取样,分别用Fenwiek＆Oostenbriak法和贝尔曼法分离土样中的孢囊和根土样中的移动线虫,并根据田间孢囊平均含卵量测算田间卵密度,用扩散系数法判断田间分布型。依据de Man法,结合雌虫阴门锥形态对比等方法进行线虫种类鉴定。结果表明,萧县禾谷类孢囊线虫为燕麦孢囊线虫（Heterodera avenae）,在田间为聚状分布,最高卵密度为42.8粒.g-1,其二龄幼虫（J2）垂直分布集中在0~100 mm土层,占该层线虫总量36.1%,J2在该层虫口密度为2.8头.g-1。在萧县等8个县市发现CCN,其中萧县70.6%调查点发现CCN。另外,在小麦根际还发现咖啡短体线虫和剑线虫等重要植物线虫。
In order to identify species and investigation of distribution of cereal cyst neamtoe（CCN） and other nematodes of wheat in Anhui Province,soils and wheat roots were sampled from wheat fields with ＂zig-zag＂ method in 22 counties.Cysts and migratory nematodes were isolated by Fenwiek Oostenbriak method and Baermmann method respectively.Identification was carried out depends on nematode morphological data measured by de Man method,and combination with comparison of morphology of female vulva cone of CCN.CCN in Xiao county was identified as Heterodera avenae,the maximum density of CCN reached 42.8 egg ·g-1 soil in Xiao county,plain distribution of CCN in field obeys clustered distribution judged by diffusion coefficient method.CCN juvenile 2 concentrated in 0~100 mm soil layer,juvenile 2 population accounts for 36.1%,and juvenile 2 density reached 2.8 head·g-1 soil in this layer.CCN widely occurs in Xiao county,where 70.6% investigation sites was found.And,other economical plant nematodes,include root lesion nematode and dagger nematode,were found in the wheat rhizosphere.
出处
《安徽农业大学学报》
CAS
CSCD
北大核心
2010年第2期189-195,共7页
Journal of Anhui Agricultural University
基金
公益性行业(农业)科技专项项目(200903040)资助
关键词
小麦
燕麦孢囊线虫
分布
根围
鉴定
wheat
cereal cyst nematode
distribution
rhizosphere
identification
