摘要
目的：研究灯盏细辛中各组分对神经母细胞瘤（SH-SY5Y）细胞的保护作用,探讨其在阿尔茨海默病（AD）治疗中的作用物质基础。方法：用柱色谱技术将灯盏细辛提取物划分为3个极性组分,并采用正交法将其配伍,以细胞MTT还原率、脂质过氧化产物（丙二醛,MDA）、神经型尼古丁受体α7蛋白表达等为活性指标,研究灯盏细辛各配伍组分对β-淀粉样肽（Aβ）损伤神经细胞的保护作用;将各配伍组分活性信息与其相应的UPLC指纹图谱化学信息进行方差分析和相关性分析研究,推测活性物质基础。结果：谱效相关性研究发现,灯盏细辛在指纹图谱中的B,C组分段具有明显活性;其中4,7-12号色谱峰与活性呈现正相关。结论：通过谱效研究,推测了灯盏细辛体外对抗Aβ神经细胞毒性的活性组分以及化学成分。为灯盏细辛深层次研究开发奠定一定的实验基础。
Objective： To investigate the neuroprotective effects of the constituents in Herba Erigerontis on neuroblastoma SH-SY5Y cells,and find out its possible material foundation in treating Alzheimer′s disease（AD）.Method： Different combinations of the three constituents in Herba Erigerontis were prepared according to the orthogonality experiment,and the indexes（MTT reduction assay,lipid peroxidation and expressions of nAChR α7 protein）were observed upon the SH-SY5Y cells followed by treatment of these combinations and β-amyloid peptide（Aβ）.The pharmacology data thus obtained and peak data in UPLC fingerprint were analyzed through ANOVA and correlationship by SPSS to give the information of active possible material foundation.Result： Constituents B and C showed clear activity and peaks of 4,7-12 did positive correlationship according to the correlation of fingerprints and pharmacology.Conclusion： This study makes a valid approach for deducing the active constituents even the exact compounds against neurotoxicity induced by Aβ by correlation of fingerprints and pharmacology.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2010年第8期1038-1041,共4页
China Journal of Chinese Materia Medica
基金
国家科技重大专项课题(2008ZX09101-021)
国际科技合作项目(2006DFA33530)
关键词
灯盏细辛
谱效关系
Β-淀粉样肽
阿尔茨海默氏病
Herba Erigerontis
constituents
fingerprints and pharmacology
β-amyloid peptide
Alzheimer′s disease
