摘要
相比于传统的单光子荧光显微镜,双光子荧光显微镜由于其具备近红外光激发、允许活体组织成像、降低光损伤和光漂白、深度组织穿透力、暗场成像及高分辨率等优点,
Two-photon absorption materials offer great promise as the new generation of fluorescent probes to image and study biological processes.We present water-dispersed two-photon functional organic nanoparticles which have been fabricated via reprecipitation method based on two-photon absorbing molecule 2,5,2′,5′-tetra(4′-N,N-diphenylaminostyryl) biphenyl(DPA-TSB) ,aiming to increase active dye loading density and enhance sensitive bioimaging in the microscopic level.In vitro confocal microscopy has demonstrated that these nanoparticles are avidly internalized into cells with bright fluorescence.The good cell membrane permeability of two-photon functional DPA-TSB nanoparticles make them use as bioimaging staining agent for the deeper tissue.
出处
《高等学校化学学报》
SCIE
EI
CAS
CSCD
北大核心
2010年第4期632-634,共3页
Chemical Journal of Chinese Universities
基金
国家自然科学基金(批准号:20834006)资助
关键词
双光子荧光
有机纳米粒子
细胞染色
Two-photon fluorescence
Organic nanoparticle
Cell stain