摘要
目的：研究莽草酸（ＳＡ）对血小板聚集性的影响及其机制，并研究其对凝血时间的影响。方法：采用比浊法测定血小板聚集功能，放射免疫法测定血小板ＴＸＢ２ 和血浆６ｋｅｔｏＰＧＦ１α水平，玻管法测定凝血时间。结果：ＳＡ 体外呈浓度依赖性抑制ＡＤＰ、胶原诱导的兔血小板聚集，ＩＣ５０ 分别为９－２５ ，３－５６ ｍ ｍｏｌ·Ｌ－１ ；在体内（ｉｐ） 抑制脑血栓模型鼠由ＡＤＰ诱导的血小板聚集；ＳＡ促进大鼠血浆６ｋｅｔｏＰＧＦ１α的生成并延长小鼠凝血时间。结论：ＳＡ 对血小板聚集及凝血系统有显著的抑制作用。
AIM: To study the inhibitory effects of shikimic acid (SA) on platelet aggregation and its mechanism, and the effect on coagulation time. METHODS: Methods of turbidimetry and radioimmunoassay were used to measure platelet aggregation and the levels of thromboxane B 2(TXB 2) and 6 keto PGF 1α . RESULTS: SA inhibited rabbit platelet aggregation induced by ADP and collagen in vitro with IC 50 of 9 25 and 3 56 mmol·L -1 , respectively. SA 25 and 50 mg·kg -1 ip for 3 d inhibited platelet aggregation induced by ADP in rats subjected to middle cerebral artery thrombosis(MCAT). SA was shown to increase rat plasma 6 keto PGF 1α and no significant effect on TXB 2 generation in PRP induced by ADP. SA 25, 50 and 100 mg·kg -1 im or iv elevated the blood coagulation time of mice. CONCLUSION: SA inhibited platelet aggregation and blood coagulation.
出处
《药学学报》
CAS
CSCD
北大核心
2000年第1期1-3,共3页
Acta Pharmaceutica Sinica
关键词
莽草酸
血小板聚集
血栓素B2
血液凝固
中药
shikimic acid
platelet aggregation
thromboxane B 2
epoprostenol
coagulation
