摘要
本文用末稍血淋巴细胞探讨了普拉托宁对人体免疫系统的影响。普拉托宁对淋巴细胞转化具有增强作用。用T细胞分裂原PHA,B细胞分裂原CowanI刺激时,对前者呈增强作用;对后者呈轻度抑制作用,在用PWM诱导Ig产生的实验系统中,经Platonin处理T细胞或B细胞,或T、B两者时,与未处理的相比,Ig分泌显著减少;而其淋转却无变化。用单克隆抗体分析T细胞亚群的结果表明,普拉托宁可使OkT8阳性T细胞比值增加。从以上结果可看出,普拉托宁可直接抑制B细胞;并可通过增强Ts/Tc的活性抑制Ig产生。
The effect ofplatonin on human peripheral bloodl ymphocytes(PBL) was studied in vitro, platonin showed proliferative effect on PBL. when PBL was stimulated with T cell mitogen phytohemagglutinin ( PHA ) and B cell mitogen Staphylococcus aureus Cowan I ( Cowan I ), it showed enhancing effect on the former and suppressive effect on the latter. In pokeweed mitogen ( PWM ) induced immunoglobulin ( Ig ) productive system,both of the T. B cells, T cell only,or B cell only were pretreated with platonin and then cocultured. The Ig secreting cells were demonstrately decreased than that of the absent platonin pretreated in any groups. However,thejH-thy midine incorporation failed to give a significant variation in any groups. In the experiment of T cell subsets detected with monoclonal antibodies and by Fluorescent Activated Cell Sorto r(FACS),the 0KT8+T cells showed an increasing proportion in the platonin treated groups. In summary. B cells were directly suppro-sjd by platonin and coicerning T cells, the T cell subsets showed a high proportion of suppressor/killer T cells, so it suggested that platonin suppressed Ig production through suppressing B cells and enhancing the proportion of suppressor/ killer T cells.
出处
《青海医学院学报》
CAS
1990年第2期7-11,共5页
Journal of Qinghai Medical College
关键词
普拉托宁
PBL
免疫功能
Platonin
Peripheral blood lymphocytes
